Marker and method for identifying single nucleotide polymorphism of bostrichthys sinensis group

A technology of single nucleotide polymorphism and Chinese snakehead, which is applied in the fields of biochemical equipment and methods, microbial determination/inspection, etc.
CN102134604AInactive Publication Date: 2011-07-27XIAMEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
XIAMEN UNIV
Publication Date
2011-07-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a marker and method for identifying single nucleotide polymorphism of a bostrichthys sinensis group and relates to a method for identifying a bostrichthys sinensis group. A single nucleotide polymorphism marker of the bostrichthys sinensis group is positioned on a 29th basic group at a 5' end of a praline tRNA gene in a bostrichthys sinensis mitochondrial genome. The method for identifying single nucleotide polymorphism of the bostrichthys sinensis group comprises the steps of: extracting a genome DNA of a bostrichthys sinensis; carrying out PCR (Polymerase Chain Reaction) amplification on the praline tRNA gene in the bostrichthys sinensis mitochondrial genome by using the DNA as a template, carrying out electrophoretic analysis on the PCR amplification products, determining that the sample is from the bostrichthys sinensis group in North of Pearl River Estuary when 330-bp PCR amplification products are tested, and determining that the sample is from the bostrichthys sinensis group in South of Pearl River Estuary when 330-bp PCR amplification products are not tested. The method for identifying single nucleotide polymorphism of the bostrichthys sinensis group is simple and easy, accurate and fast, impartial and subjective as well as economical and practical.
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Description

technical field

[0001] The invention relates to a method for identifying a population of Channa sinensis, in particular to a single nucleotide polymorphism marker and a method for identifying a population of Channa sinensis. Background technique

[0002] Chinese snakehead (Bostrychus sinensis), also known as mullet, salmon, bamboo shoot shell fish, earth fish, crab tiger, belongs to Perciformes (Perciformes), Gobioidei (Gobioidei), pond snakehead family (Eleotridae) , Wutang Channa (Bostrychus), whose wild populations are widely distributed in the intertidal sea area from the south of the Yangtze River Estuary to Southeast Asia, is a rare and valuable seafood in the southeast coast of my country. At present, the market price can reach 160 yuan / kg (Zhang Jiandong , the growth, growth model and life history types of Channa sinensis. Chinese Journal of Ecology [J], 200222(6): 841-846). At present, Channa sinensis is cultivated in Guangdong, Guangxi, Fujian, and Zhejiang, but the...

Claims

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