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Culture solution for differentiating adipose-derived stromal cells into myocardial cells and preparation method thereof

A technology of stromal cells and cardiomyocytes, applied in the biological field, can solve problems such as the difficulty in the clinical application of the induction system, the difficulty in differentiating into key components of cardiomyocytes, and the impact on clinical application

Inactive Publication Date: 2011-08-03
NORTHWEST A & F UNIV
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Problems solved by technology

[0004] Although the above-mentioned culture systems can obtain cardiomyocytes with spontaneous contraction function, they also have the following problems: ①The complex culture system makes it difficult to divide the effective induction components, which is the key to further refine the differentiation of adipose stromal cells into cardiomyocytes in vitro ② complex additives may directly affect the basic biological characteristics of the generated cardiomyocytes, and then affect their clinical application; ③ in vitro co-culture induction system with cardiomyocytes is difficult in clinical application; ④ additives expensive

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  • Culture solution for differentiating adipose-derived stromal cells into myocardial cells and preparation method thereof
  • Culture solution for differentiating adipose-derived stromal cells into myocardial cells and preparation method thereof

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Embodiment Construction

[0021] The present invention will be described in detail below in conjunction with specific embodiments.

[0022] The scheme for the present invention to achieve the above object is: a preparation method for a culture medium in which adipose stromal cells differentiate into cardiomyocytes, which is characterized in that: the contents of each component are as follows: Gibco DMEM high-sugar medium: 10g / L; NaHCO 3 : 2.3g / L; HEPES: 2.6g / L; Gibco fetal bovine serum: 15~25% (volume percentage); Penicillin: 5×10 4 U / L; Streptomycin: 5×10 4 U / L; Deionized water: 75-85% (volume percentage). The preparation method is as follows: ① Sterilize deionized water at 120°C for 30 minutes, and cool to room temperature naturally; ② Dilute Gibco DMEM high-sugar medium, NaHCO 3 , HEPES, Gibco fetal bovine serum, penicillin and streptomycin were poured into ① sterilized deionized water, and stirred evenly with a magnetic stirrer; ③ Filter ② through 0.45 μm and 0.22 μm sterile microporous membranes...

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Abstract

The invention discloses a method for preparing culture solution for differentiating adipose-derived stromal cells into myocardial cells. 1L of culture solution comprises the following components: 10g of Gibco DMEM(Dulbecco's Modified Eagle Medium) high glucose medium, 2.3g of NaHCO3, 2.6g of N-2-hydroxyethylpiperazine-N-ethane-sulphonicacid (HEPES), 15 to 25 volume percent of Gibco fetal calf serum, 5*104U of penicillin, 5*104U of streptomycin and 75 to 85 volume percent of deionized water. A simple and definite culture solution system for differentiating the adipose-derived stromal cells into the myocardial cells is obtained, and meets higher application security standard.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a culture medium for differentiating adipose stromal cells into cardiomyocytes and a preparation method thereof. Background technique [0002] The culture medium for adipose stromal cells to differentiate into cardiomyocytes is a culture system that induces stromal cells derived from adipose tissue or adult stem cells to differentiate into cardiomyocytes with spontaneous contraction function in vitro. Due to the increasing use of adult stem cells in medical clinical tissue repair Therefore, the value of this system in scientific research and clinical application is also increasingly showing its importance. The additives in the culture medium, the type and concentration of serum, and the pH value of the culture medium all determine the degree of differentiation of stromal cells or stem cells into functional cardiomyocytes. In addition, the additives also affect the molecular pathway ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077
Inventor 杨公社赵丽丽鞠大鹏高倩王二云史新娥郑雪莉
Owner NORTHWEST A & F UNIV
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