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Method for establishing high-pressure liquid-phase fingerprinting of evodia and extractives thereof

A technology for fingerprinting and establishing a method, applied in the field of drug analysis, can solve the problems of a small number of characteristic peaks, and there is no comprehensive reflection of the fingerprinting technology of Evodia Fructus extracts, etc., and achieves good separation, high quality control precision, and reproducibility. Good results

Active Publication Date: 2011-08-03
贵州中医药大学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the fat-soluble and water-soluble fingerprint technology of Evodia rutaecarpa (public number: CN1932506A, CN1932507A) has been studied, but the fingerprint technology has only made fat-soluble, water-soluble The water-soluble part is studied, and the number of characteristic peaks is small. There is no research technology that fully reflects the fingerprints of the complete components of Evodia rutaecarpa and its extract in the GAP base in Yuqing, Guizhou.

Method used

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  • Method for establishing high-pressure liquid-phase fingerprinting of evodia and extractives thereof
  • Method for establishing high-pressure liquid-phase fingerprinting of evodia and extractives thereof
  • Method for establishing high-pressure liquid-phase fingerprinting of evodia and extractives thereof

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Embodiment 1

[0033] Embodiment 1, the method for establishing the HPLC fingerprint of medicinal material Evodia rutaecarpa

[0034] 1 Instruments, samples, reagents and reagents

[0035] 1.1 Instrument: Agilent 1100 high performance liquid chromatograph, DAD detector, Agilent Chemstation chromatographic workstation, chromatographic column: Diamonsil C 18 Column (250 mm x 4.6 mm, 5 μm).

[0036] 1.2 Samples: 21 batches of Evodia rutaecarpa (Juss.) Benth.

[0037] 1.3 Reagents: Methanol is chromatographically pure, water is pure water, and other reagents are analytically pure.

[0038] 2 Methods and results

[0039] 2.1 Preparation of the test solution

[0040]Take about 0.2 g of the sample powder (passed through a 60-mesh sieve) dried to constant weight, accurately weigh it, put it in a 50 mL conical flask with a stopper, add 25 mL of 50% methanol, weigh, and ultrasonically process (power 250W, frequency 33kHz) for 20 Minutes, let cool, weigh, make up for the lost weight, filter, take ...

Embodiment 2

[0047] Example 2, Method for Establishing HPLC Fingerprint of Evodia Evodia Extract

[0048] 1 Instruments, samples, reagents and reagents

[0049] 1.1 Instrument: Agilent 1100 high performance liquid chromatograph, DAD detector, Agilent Chemstation chromatographic workstation, chromatographic column: ZORBAX Eclipse XDB-C 18 (4.6×250mm, 5μm).

[0050] 1.2 Sample: The raw medicinal material of Evodia rutaecarpa (Juss.) Benth.var.bodinieri (Dode) Huang produced in Yuqing, Guizhou is the dry and nearly mature fruit of Evodiarutaecarpa (Juss.) Benth.var.bodinieri (Dode) Huang.

[0051] 1.3 Reagents: Acetonitrile is chromatographically pure, water is pure water, and other reagents are analytically pure.

[0052] 2 Methods and Results

[0053] 2.1 Preparation of Evodia Evodia Extract:

[0054] 2.1.1 Preparation of water extract of Evodia rutaecarpa: Weigh 20 g of Evodia rutaecarpa, reflux extraction with 10 times the amount of water for 3 times, 2 hours each time, combine the fi...

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Abstract

The invention provides a method for establishing the HPLC (High Performance Liquid Chromatography) fingerprinting quality of a GAP (Good Aquaculture Practices) evodia material and extractives thereof in Yuqing, Guizhou, and the method comprises the following steps: preparing a sample solution to be tested; preparing the extractives; determining chromatography conditions; utilizing an HPLC to measure; selecting two counterfeit drugs (pepper and coriaria sinica) of the evodia for the first time to finish the applicability inspection of the fingerprinting; obtaining the HPLC fingerprinting of the evodia material and the extractives thereof; expressing the integral chemical information of the evodia material and the extractives thereof more comprehensively; and determining the finger characteristics of the evodia material and the extractives thereof. The fingerprinting comprises a water solubility part and a lipid solubility part, has the characteristics of simple and convenient method, good repeatability, multiple characteristic peaks, accuracy and reliability. The established HPLC fingerprinting can be used for effectively controlling the quality of the evodia material and the extractives thereof and identifying truth or falsity.

Description

technical field [0001] The invention relates to a method for establishing the HPLC (high performance liquid chromatography) fingerprint of the medicinal material Evodia rutaecarpa and its extract from Yuqing, Guizhou, and the HPLC standard fingerprint of the medicinal material Evodia rutaecarpa and its extract obtained therefrom, belonging to the technical field of drug analysis. Background technique [0002] Evodia rutaecarpa (Juss.) Benth., Evodiarutaecarpa (Juss.) Benth.var.officinalis (Dode) Huang or Evodiarutaecarpa (Juss.) Benth.var.bodinieri (Dode) Huang Dry nearly ripe fruit. It was first recorded in "Shen Nong's Materia Medica", and it is mainly produced in Guizhou, Hunan, Chongqing, Jiangxi, Zhejiang and other places. Evodia rutaecarpa is a well-known authentic medicinal material and commonly used Miao medicine in Guizhou Province, accounting for more than 30% of the total national output. According to the standardization requirements of GAP, Yuqing, Guizhou has c...

Claims

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Application Information

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IPC IPC(8): G01N30/36A61K36/754
Inventor 张丽艳罗君杨玉琴张丽丽陈新华李健何平王晖潘雯婷黄健
Owner 贵州中医药大学
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