Method for establishing high-pressure liquid-phase fingerprinting of evodia and extractives thereof

A technology for fingerprinting and establishing a method, applied in the field of drug analysis, can solve the problems of a small number of characteristic peaks, and there is no comprehensive reflection of the fingerprinting technology of Evodia Fructus extracts, etc., and achieves good separation, high quality control precision, and reproducibility. Good results

Active Publication Date: 2011-08-03
贵州中医药大学
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the fat-soluble and water-soluble fingerprint technology of Evodia rutaecarpa (public number: CN1932506A, CN1932507A) has been studied, but the fingerprint technology has only made fat-soluble, water-soluble The water-so

Method used

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  • Method for establishing high-pressure liquid-phase fingerprinting of evodia and extractives thereof
  • Method for establishing high-pressure liquid-phase fingerprinting of evodia and extractives thereof
  • Method for establishing high-pressure liquid-phase fingerprinting of evodia and extractives thereof

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0033] Example 1. Establishing method of HPLC fingerprint of Evodia officinalis

[0034] 1 Instruments, samples, reagents and reagents

[0035] 1.1 Instrument: Agilent 1100 high performance liquid chromatograph, DAD detector, Agilent Chemstation chromatography workstation, chromatographic column: Diamonsil C 18 Column (250mm×4.6mm, 5μm).

[0036] 1.2 Samples: 21 batches of Evodia rutaecarpa (Juss.) Benth., from Guizhou, Hunan, Chongqing, Jiangxi, Zhejiang and other places, were identified as the fruits of Evodia rutaecarpa (Juss.) Benth.

[0037] 1.3 Reagents: methanol is chromatographically pure, water is pure water, and the remaining reagents are analytical pure.

[0038] 2 methods and results

[0039] 2.1 Preparation of test solution

[0040] Take about 0.2g of the sample powder (passed through a 60-mesh sieve) dried to constant weight, accurately weigh it, place it in a 50mL conical flask with stopper, add 25mL of 50% methanol, weigh, and ultrasonically treat it (power 250W, frequency...

Example Embodiment

[0047] Example 2: Establishing method of HPLC fingerprint of Evodia Fructus extract

[0048] 1 Instruments, samples, reagents and reagents

[0049] 1.1 Instrument: Agilent 1100 high performance liquid chromatograph, DAD detector, Agilent Chemstation chromatography workstation, chromatographic column: ZORBAX Eclipse XDB-C 18 (4.6×250mm, 5μm).

[0050] 1.2 Sample: The original medicinal material of the evodia extract sample is the dry and nearly mature fruit of Evodiarutaecarpa (Juss.) Benth.var.bodinieri (Dode) Huang from Yuqing, Guizhou.

[0051] 1.3 Reagents: Acetonitrile is chromatographically pure, water is pure water, and the rest of the reagents are analytical pure.

[0052] 2 methods and results

[0053] 2.1 Preparation of evodia extract:

[0054] 2.1.1 Preparation of water extract of Evodia: Weigh 20g of Evodia and extract 3 times with 10 times the amount of water under reflux for 2 hours each time. Combine the filtrate, place in an evaporating dish, and steam to a thick paste with...

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Abstract

The invention provides a method for establishing the HPLC (High Performance Liquid Chromatography) fingerprinting quality of a GAP (Good Aquaculture Practices) evodia material and extractives thereof in Yuqing, Guizhou, and the method comprises the following steps: preparing a sample solution to be tested; preparing the extractives; determining chromatography conditions; utilizing an HPLC to measure; selecting two counterfeit drugs (pepper and coriaria sinica) of the evodia for the first time to finish the applicability inspection of the fingerprinting; obtaining the HPLC fingerprinting of the evodia material and the extractives thereof; expressing the integral chemical information of the evodia material and the extractives thereof more comprehensively; and determining the finger characteristics of the evodia material and the extractives thereof. The fingerprinting comprises a water solubility part and a lipid solubility part, has the characteristics of simple and convenient method, good repeatability, multiple characteristic peaks, accuracy and reliability. The established HPLC fingerprinting can be used for effectively controlling the quality of the evodia material and the extractives thereof and identifying truth or falsity.

Description

technical field [0001] The invention relates to a method for establishing the HPLC (high performance liquid chromatography) fingerprint of the medicinal material Evodia rutaecarpa and its extract from Yuqing, Guizhou, and the HPLC standard fingerprint of the medicinal material Evodia rutaecarpa and its extract obtained therefrom, belonging to the technical field of drug analysis. Background technique [0002] Evodia rutaecarpa (Juss.) Benth., Evodiarutaecarpa (Juss.) Benth.var.officinalis (Dode) Huang or Evodiarutaecarpa (Juss.) Benth.var.bodinieri (Dode) Huang Dry nearly ripe fruit. It was first recorded in "Shen Nong's Materia Medica", and it is mainly produced in Guizhou, Hunan, Chongqing, Jiangxi, Zhejiang and other places. Evodia rutaecarpa is a well-known authentic medicinal material and commonly used Miao medicine in Guizhou Province, accounting for more than 30% of the total national output. According to the standardization requirements of GAP, Yuqing, Guizhou has c...

Claims

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Application Information

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IPC IPC(8): G01N30/36A61K36/754
Inventor 张丽艳罗君杨玉琴张丽丽陈新华李健何平王晖潘雯婷黄健
Owner 贵州中医药大学
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