Fingerprint detection method of Danshu capsule

A technology of Danshu capsules and fingerprints, which is applied in the field of fingerprint detection of Danshu capsules, can solve the problems of long detection time, poor separation effect, and few detected components, and achieve short detection time, saving detection costs, and high resolution Good results

Active Publication Date: 2016-04-06
SICHUAN JISHENGTANG PHARMLS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve technical problems such as long detection time, few detected components, and poor separation effect in the prior ar

Method used

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  • Fingerprint detection method of Danshu capsule
  • Fingerprint detection method of Danshu capsule
  • Fingerprint detection method of Danshu capsule

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0027] Example 1 Gas Chromatographic Detection of Danshu Capsule

[0028] Sample preparation method: Take 0.72g of the contents of Danshu Capsule, place it in a test tube with stopper, add 10ml of absolute ethanol, fully shake or ultrasound and then stand for 20min, filter, and take the filtrate to get it;

[0029] Contrast chromatographic conditions: initial temperature 60℃, keep for 4min, 2℃·min -1 Increase the temperature to 100℃ at a rate of 100℃·min -1 The rate of heating is 230℃, the temperature of the injection port is 250℃, the FID detector, the detector temperature is 250℃, the injection volume is 1.0μl, the split ratio is 10:1, carrier gas: N2, flow rate 20ml*min -1 .

[0030] Prepare the sample solution according to the above method and perform gas chromatography test. The test results are as follows figure 1 As shown, there are 13 chromatographic peaks detected, and the retention times are 0.60min, 0.76min, 1.10min, 3.44min, 4.40min, 5.08min, 5.59min, 7.63min, 8.05min, 9.3...

Example Embodiment

[0031] Example Erdanshu capsule gas chromatography detection

[0032] Sample preparation method: Take 0.72g of the contents of Danshu Capsule, place it in a test tube with stopper, add 10ml of absolute ethanol, fully shake or ultrasound and then stand for 20min, filter, and take the filtrate to get it;

[0033] Chromatographic conditions: initial temperature 73℃, keep 4min, 2℃·min -1 Increase the temperature to 100℃ at a rate of 6℃·min -1 The rate of heating is 160℃, the temperature of the injection port is 250℃, the FID detector, the detector temperature is 250℃, the injection volume is 1.0μl, the split ratio is 40:1, the carrier gas: N2, the flow rate is 2ml*min -1 .

[0034] Prepare the sample solution according to the above method and perform gas chromatography test. The test results are as follows figure 2 As shown, the total number of detected component chromatographic peaks is 20, and the retention times are 2.07min, 2.51min, 3.28min, 3.44min, 7.44min, 9.40min, 9.82min, 10.37m...

Example Embodiment

[0035] Example Sandanshu capsule gas chromatography detection

[0036] Sample preparation method: Take 0.72g of the contents of Danshu Capsule, place it in a test tube with stopper, add 10ml of absolute ethanol, fully shake or ultrasound and then stand for 20min, filter, and take the filtrate to get it;

[0037] Chromatographic conditions: initial temperature 74℃, keep 4min, 2℃·min -1 Increase the temperature to 100℃ at a rate of 6℃·min -1 The rate of heating is 200℃, the temperature of the injection port is 250℃, the FID detector, the detector temperature is 250℃, the injection volume is 1.0μl, the split ratio is 40:1, carrier gas: N2, flow rate 20ml*min-1 , Carrier gas: N2, flow rate 2ml*min -1 .

[0038] Prepare the sample solution according to the above method and perform gas chromatography test. The test results are as follows image 3 As shown, the total number of detected component chromatographic peaks is 20, and the retention times are 2.05min, 2.47min, 3.21min, 3.37min, 7.2...

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Abstract

The present invention provides a fingerprint detection method of Danshu capsule. The method using a gas chromatography detection method with the initial temperature of 73 DEG C-75 DEG C, and the terminal temperature of 160 DEG C-200 DEG C; programming temperature rises is employed; the inlet temperature is at 250 DEG C, and the detector temperature is at 250 DEG C; and a sample solution prepared from Danshutong capsule contents is detected. The detection method can detect a plurality of components, has good separation on the components, can provide effective standards for quality control of Danshutong capsule; the overall peak time is short, thereby greatly reducing the detection time and cost; and the method is more conducive to industrial applications.

Description

technical field [0001] The invention relates to the field of drug detection, in particular to a fingerprint detection method of Danshu capsules. Background technique [0002] Mint is widely distributed in various parts of my country, such as Yunnan, Jiangsu, Zhejiang, Jiangxi, etc. are all produced. China is one of the main export countries of peppermint oil and menthol. Peppermint oil (OleumMenthaeDementholatum) is the main chemical component in Peppermint. It is a traditional Chinese medicine, which has the effects of cooling and relieving itching, anti-inflammatory and analgesic, expectorant, choleretic, and stone-dissolving [Liu Hongjie et al., Peppermint Oil Research Progress, Shandong University of Traditional Chinese Medicine Journal 2006, 30(6): 502-505], mainly used clinically to treat chronic cholecystitis [Han Jie et al., Clinical Observation of 25 Cases of Chronic Cholecystitis Treated by Danshu Capsules, Chinese Patent Medicine, 2007, 29(1): instruction manual...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 柯潇邬智刚白晓春
Owner SICHUAN JISHENGTANG PHARMLS
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