Ginsenoside Rg1 molecular imprinting material and preparation method and application thereof

A technology of molecular imprinting and ginsenoside, which is applied in chemical instruments and methods, ion exchange, and other chemical processes, can solve problems such as poor selectivity, inactivation of chiral configuration, and difficulty in separation and purification, and achieve good recognition performance. Effect

Inactive Publication Date: 2011-08-31
MINZU UNIVERSITY OF CHINA
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Problems solved by technology

[0003] (2) The ingredients contained in Chinese herbal medicine are complex. In order to improve the therapeutic effect of Chinese herbal medicine, it is necessary to extract the active ingredients as much as possible and remove the ineffective and toxic ingredients.
The separation and extraction of active ingredients of traditional Chinese medicine often use silica gel column, C 18 Bonded phase column, Al 2 o 3 Columns, etc. use the difference in the ads

Method used

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  • Ginsenoside Rg1 molecular imprinting material and preparation method and application thereof
  • Ginsenoside Rg1 molecular imprinting material and preparation method and application thereof

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Embodiment 1

[0020] 1. Preferred preparation method of Rg1 molecularly imprinted material:

[0021] The mass of Rg1 is 1-50 mg, the functional monomer is 50-560 mg acrylamide and 5-590 mg methacrylic acid, and the cross-linking agent EDMA 1000-5500 mg is mixed and dissolved in a round-bottomed flask filled with 5-80 mL porogen acetonitrile solution , add initiator AIBN 78mg, pass nitrogen gas for deoxygenation for 15min, and seal quickly under the protection of nitrogen gas. The sealed flask was placed in an ice-water bath at 0°C and irradiated with ultraviolet rays for 24 hours to obtain the material. After the reacted material was repeatedly rinsed with deionized water, it was washed repeatedly with a 0.2mol / L sodium hydroxide solution of 10% (W / V) sodium dialkylsulfate (SDS) to remove imprinted molecules, and washed with deionized water After reaching neutrality, use methanol as a solvent to elute with a Soxhlet extractor to remove incompletely reacted organic reagents, and check the e...

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Abstract

The invention discloses a preparation method of a ginsenoside Rg1 molecular imprinting material, and a method for separating and purifying an Rg1 mimetic in a natural product by using the material as a solid-phase extracting agent. The material belongs to novel separating materials, can serve as a fixing phase of a solid-phase extraction column, is used for quickly, effectively and directionally separating and gathering the Rg1 mimetic from the Chinese medicine to obtain a target component with high extraction rate, and has extremely high application value.

Description

Technical field: [0001] The patent of the present invention relates to the field of synthesis and its application in targeted separation, and specifically relates to the preparation of ginsenoside Rg1 molecularly imprinted material and the method of using the material as a solid phase extraction agent to separate and purify Rg1 mimics in plants. Background technique: [0002] (1) Ginsenoside Rg1 has the effect of promoting the proliferation of neural stem cells, and plays an important role in the treatment of stroke sequelae, neurodegenerative diseases and other diseases. In view of the lack of clinical drugs that promote the proliferation of neural stem cells, it is of great significance to use Rg1 as a template to synthesize molecularly imprinted materials for targeted screening of active ingredients from traditional Chinese medicines, ethnic medicines, and natural plants. [0003] (2) The ingredients contained in Chinese herbal medicine are complex. In order to improve th...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/30B01D15/08
Inventor 刘庆山尹小英黄火强徐羽谷雨龙刘伟志方亮
Owner MINZU UNIVERSITY OF CHINA
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