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Preparation method of antioxidant in blueberry leaves

An antioxidant substance, blueberry leaf technology, applied in the field of preparation of natural antioxidant substances, can solve the problems of large environmental impact, low purity, complicated process, etc., and achieve the effect of strong antioxidant activity, good purity, and simple process

Active Publication Date: 2013-04-10
海宁市袁花镇工业投资有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the technical defects of large impact on the environment, complex process, high cost and low extraction purity in the above process, the purpose of the present invention is to provide a method for extracting proanthocyanidins from blueberry leaves, which is simple in process and environmentally friendly , good purity, product safety and strong antioxidant activity

Method used

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  • Preparation method of antioxidant in blueberry leaves

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Collect fresh blueberry leaves, rinse with tap water, and drain.

[0018] 2. Cut 100 g of the above-mentioned blueberry leaves into pieces about 1 cm in length, add 800 ml (400 ml + 400 ml) of tap water, adjust the pH to 8, and extract twice in a 90°C water bath for 1.5 h and 1 h respectively. .

[0019] 3. Take the filtrate by filtration and concentrate under reduced pressure (50°C) to about 50 ml.

[0020] 4. Absorb on AB-8 macroporous resin, elute with water and 10-30% ethanol, detect with ultraviolet spectrophotometry, collect the eluate with strong anti-oxidation activity that absorbs at 280nm, and concentrate under reduced pressure (50°C) to a paste.

[0021] 5. Vacuum-dried (50°C) to obtain 2.97 g of light brown powder. Using the sulfuric acid-vanillin method, the proanthocyanidin standard was used as a calibration curve to calculate the proanthocyanidin content of the sample as 95.3%.

Embodiment 2

[0023] 1. Collect fresh blueberry leaves, rinse with tap water, and drain.

[0024] 2. Cut 80 g of the above-mentioned blueberry leaves into pieces about 1 cm in length, add 1000ml (400 ml + 300 ml + 300 ml) of tap water, adjust the pH to 6, and extract in a boiling water bath for 3 times for 2.5 h, 2 h and 1.5 h.

[0025] 3. Take the filtrate by filtration, concentrate under reduced pressure (50°C) to about 40 ml, which is about 25 times the original concentration.

[0026] 4. Absorb on AB-8 macroporous resin, elute with water and 10-30% ethanol, detect with ultraviolet spectrophotometry, collect the eluate with strong anti-oxidation activity that absorbs at 280nm, and concentrate under reduced pressure (50°C) to a paste.

[0027] 5. Vacuum-dried (50°C) to obtain 2.52 g of light brown powder. Using the sulfuric acid-vanillin method, the proanthocyanidin standard was used as a calibration curve to calculate the proanthocyanidin content of the sample as 95.5%.

Embodiment 3

[0029] 1. Collect fresh blueberry leaves, rinse with tap water, and drain.

[0030] 2. Cut 80 g of the above-mentioned blueberry leaves into pieces about 1 cm in length, add 560 ml of tap water, adjust the pH to 4, extract in a boiling water bath for 2 hours, filter, add 560 ml of tap water to the filter residue, extract in a boiling water bath for 1.5 h, and filter.

[0031] 3. Combine the filtrates and concentrate under reduced pressure (50°C) to about 55 ml, which is about 20 times the original concentration.

[0032] 4. Absorb on AB-8 macroporous resin, elute with water and 10-30% ethanol, detect with ultraviolet spectrophotometry, collect the eluate with strong anti-oxidation activity that absorbs at 280nm, and concentrate under reduced pressure (50°C) to a paste.

[0033] 5. Vacuum drying (50°C) to obtain 2.28 g of light brown powder. Using the sulfuric acid-vanillin method, the proanthocyanidin standard was used as a standard curve to calculate the proanthocyanidin con...

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Abstract

The invention belongs to a preparation technology of natural antioxidant, particularly relates to a preparation method of antioxidant in blueberry leaves. The preparation method of the antioxidant in the blueberry leaves includes the steps as follows: (1) collecting fresh blueberry leaves, washing the fresh blueberry leaves with water, draining away water, shearing the leaves into segments with different length, adding 8-14 times water, adjusting pH to 3-8, conducting extraction in hot-water bath at the temperature of 90-100 DEG C, filtering the extract, and decompressing and concentrating the extract to 15-25 times of original concentration at the temperature of 50-60 DEG C; and (2) conducting adsorption with macroporous resin, eluting with water and 10%-30% alcohol, detecting by ultraviolet spectrophotometry, collecting adsorbed eluent with strong antioxidant activity at 280nm part, decompressing and concentrating the collected eluent to paste, and conducting vacuum-drying at the temperature 50-60 DEG C, thus obtaining light brown powder. The preparation method is simple in technology, friendly to environment and good in purity; and the product is safe and has strong antioxidantactivity.

Description

technical field [0001] The invention belongs to the preparation process of natural antioxidant substances, in particular to a preparation method of antioxidant substances in blueberry leaves. Background technique [0002] With the development of the economy and the improvement of people's living standards, people pay more and more attention to the safety of food. Antioxidation is a key issue in food processing. At present, chemically synthesized antioxidants such as di-tert-butylhydroxytoluene (BHT) and tert-butylhydroxyanisole (BHA) are mainly used. Since chemically synthesized antioxidants are more or less harmful to the human body, people are increasingly inclined to replace synthetic antioxidants with natural antioxidants. Therefore, research and development of efficient and safe natural antioxidants has become a major issue in today's food science research. hot spots, and many plants are rich in antioxidant active ingredients, which are good sources of natural antioxid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L3/3472
Inventor 徐丽珊黄启亮陈文荣
Owner 海宁市袁花镇工业投资有限公司
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