Method for preparing fermentation malt extract for cigarettes
A malt extract and fermented technology, which is applied in the field of preparation of fermented malt extract for tobacco, can solve the problems such as the inability to produce malt extract for tobacco, achieve the purpose of increasing fragrance and soft smoke, increasing categories, reducing stimulating effect
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Embodiment 1
[0022] 1) Culture medium preparation
[0023] a. Raw material pretreatment: malt and water were refluxed and extracted for 1 hour according to the weight ratio of 1:5, filtered and concentrated to a relative density of 1.02 to obtain a malt extract;
[0024] b. Adding glucose: adding 0.1% glucose by weight to the prepared malt extract to prepare the culture medium;
[0025] 2) medium sterilization
[0026] Sterilize the medium at 115°C for 15 minutes;
[0027] 3) Vaccination
[0028] Under sterile conditions, inoculate the active dry yeast of Saccharomyces cerevisiae into the medium, and the inoculation amount is 0.01%;
[0029] 4) fermentation
[0030] When the pH value is 2.5, the stirring speed is 200rpm, the ventilation volume is 1VVM, and the fermentation temperature is 15°C, ferment for 9 days;
[0031] 5) Separation
[0032] Filter the fermented liquid obtained after fermentation to remove impurities, take the clarified liquid, concentrate to 1 / 2 of the original v...
Embodiment 2
[0034] 1) Culture medium preparation
[0035] a. Raw material pretreatment: malt and water were refluxed and extracted for 5 hours according to the weight ratio of 1:15, filtered, and concentrated to a relative density of 1.10 to obtain a malt extract;
[0036] b. Adding glucose: adding 5% glucose by weight to the prepared malt extract to prepare the culture medium;
[0037] 2) medium sterilization
[0038] Sterilize the medium at 115°C for 30 minutes;
[0039] 3) Vaccination
[0040] Under sterile conditions, inoculate the active dry yeast of Saccharomyces cerevisiae into the medium, and the inoculum amount is 0.5%;
[0041] 4) fermentation
[0042] The pH value is 3.5, the stirring speed is 200rpm, the ventilation volume is 1VVM, and the fermentation temperature is 30°C for 3 days;
[0043] 5) Separation
[0044] Filter the fermented liquid obtained after fermentation to remove impurities, take the clarified liquid, concentrate it to 1 / 5 of the original volume, add 95%...
Embodiment 3
[0046] 1) Culture medium preparation
[0047] a. Raw material pretreatment: malt and water were refluxed and extracted for 3 hours according to the weight ratio of 1:10, filtered, and concentrated to a relative density of 1.12 to obtain a malt extract;
[0048] b. Adding glucose: adding 2% glucose by weight to the prepared malt extract to prepare the culture medium;
[0049] 2) medium sterilization
[0050] Sterilize the medium at 115°C for 20 minutes;
[0051] 3) Vaccination
[0052] Under sterile conditions, inoculate the active dry yeast of Saccharomyces cerevisiae into the medium, and the inoculation amount is 0.1%;
[0053] 4) fermentation
[0054] When the pH value is 5.5, the stirring speed is 200rpm, the ventilation volume is 1VVM, and the fermentation temperature is 20°C, ferment for 5 days;
[0055] 5) Separation
[0056] Filter the fermented liquid obtained after fermentation, remove impurities, take the clarified liquid, concentrate to 1 / 3 of the original vol...
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