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Cross protection vaccine

A technology of cross-protection and cross-immunity, which is applied in the field of vaccinology, can solve the problems of single vaccines and achieve the effect of simple preparation and high-efficiency cross-protection effect

Inactive Publication Date: 2011-09-14
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some vaccines have been reported that are effective under laboratory conditions, these vaccines are mainly monovaccine, that is, active against only one pathogen (Edwardella tarda or Vibrio harveyi)
Vaccines capable of simultaneously targeting Edwardsiella tarda and Vibrio harveyi, that is, cross-protective vaccines, have not yet been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Vaccine construction:

[0013] Plasmid pAQ1 (see Zhang W, Sun K, Cheng S, Sun L.Characterization of DegQ for its construction process Vh , a serine protease and a protective immunogen from a pathogenic Vibrio harveyi strain. Appl Environ Microbiol 2008; 74: 625462.) was transformed into lager by the Hanahan method (Sambrook and Russell: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press 2001). Edwardsiella ATCC15947 (purchased from ATCC, USA) was obtained from strain Et15VhD, which is a vaccine strain with cross-protective effect on Edwardsiella tarda and Vibrio harveyi.

Embodiment 2

[0015] Vaccine application

[0016] Step 1) Preparation of vaccine preparation liquid. Cultivate the vaccine strain Et15VhD obtained above to OD in LB liquid medium 600 0.8-1, then centrifuge the culture solution (5000g, 4°C, 10min), collect the bacteria, suspend it in PBS to a final concentration of 2x10 8 cfu / ml is the vaccine preparation solution.

[0017] The LB composition is by weight percentage: 1.0% peptone, 0.5% yeast powder, 1.0% sodium chloride, 97.5% distilled water; the PBS composition is by weight percentage: 0.8% NaCl, 0.02% KCl, 0.358% Na 2 HPO 4 .12H 2 O, 0.024% NaH 2 PO 4 , 98.798% distilled water.

[0018] Step 2) Vaccination. 120 flounder (each weighing about 12 g) were randomly divided into 4 groups, 30 in each group. These 4 groups are named A, B, C and D respectively. Each fish in groups A and C was injected intraperitoneally with 100ul of the vaccine preparation solution in step 1) above, and each fish in groups B and D (control group) was inj...

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Abstract

The invention relates to the field of vaccinology, in particular to a cross protection vaccine specific to Edwardsiella tarda and Vibrio harveyi. The cross protection vaccine is a recombination bacterial strain that recombines and converts plasmid pAQ1 to Edwardsiella tarda ATCC15947. The cross protection vaccine is a cross protection vaccine of Edwardsiella tarda and Vibrio harveyi. The vaccine has over 90% protective effect on infection of Edwardsiella tarda and Vibrio harveyi.

Description

technical field [0001] The invention relates to the field of vaccinology, in particular to a cross-protection vaccine against Edwardsiella tarda and Vibrio harveyi. Background technique [0002] Edwardsiella tarda and Vibrio harveyi are Gram-negative bacteria widely distributed in various water environments. These two bacteria are important aquaculture pathogens capable of infecting a variety of marine and freshwater fish species. In my country, the infection of Edwardsiella tarda and Vibrio harveyi is one of the main causes of economic loss of a variety of cultured fish. In addition to fish, Edwardsiella tarda can also infect humans, while Vibrio harveyi is an important pathogen of farmed shrimp, which can cause luminous vibriosis with high mortality. Currently, there are no commercially available vaccines against Edwardsiella tarda and Vibrio harveyi. Although some vaccines effective under laboratory conditions have been reported, these vaccines are mainly monovaccine, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/116A61K39/106A61K39/02C12N1/21A61P31/04
Inventor 孙黎胡永华
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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