Human-derived voltage-gated potassium channel 1.3 immunogenic peptide fragment and application thereof

A voltage-gated, immunogenic technology, applied in the direction of anti-animal/human immunoglobulins, peptides, allergic diseases, etc., can solve the problems of blocking Kv1.3 channel blockers, arrhythmias, etc.

Inactive Publication Date: 2011-09-14
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Description
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Problems solved by technology

Studies have found that the binding sites of Kv1.3 channels and cardiac repolarization HERG and KCNQ1 / KCNE1 potassium channel blockers are highly conserved, which makes some existing Kv1.3 channel blockers block Kv1.3 channels at the same time , and also block HERG and KCNQ1 / KCNE1 channels, often leading to the occurrence of drug-induced arrhythmias, which hinders the further development of some Kv1.3 channel blockers into clinical drugs

Method used

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  • Human-derived voltage-gated potassium channel 1.3 immunogenic peptide fragment and application thereof
  • Human-derived voltage-gated potassium channel 1.3 immunogenic peptide fragment and application thereof
  • Human-derived voltage-gated potassium channel 1.3 immunogenic peptide fragment and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] Anti-hKv1.3 extracellular loop peptide E314 antibody inhibits hKv1.3 current stably expressed in HEK293 cell line—a new and specific hKv1.3 channel inhibitor

[0029] Materials and Methods

[0030] 1.1 Experimental objects

[0031] Male New Zealand white rabbits, purchased from the Experimental Animal Center of Tongji Medical College, Huazhong University of Science and Technology, clean grade, weighing 2-3 kg, were randomly divided into immunization group and sham immunization group. Human atrial and ventricular papillary muscle samples were obtained from patients undergoing surgery for congenital heart disease and valvular disease in cardiac surgery. There were 32 males and 38 females, aged (66.3±1.4) years old. The experimental protocol complied with the principles and terms stated in the "Declaration of Helsinki" (revised in 2008), and was approved by the Ethics Committee of Huazhong University of Science and Technology. Human embryonic kidney cells (HEK293) were p...

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Abstract

The invention provides a human-derived voltage-gated potassium channel 1.3 immunogenic peptide fragment and applications thereof. The peptide is composed of 13 continuous amino acids on an extracellular loop between an alpha subunit transmembrane fragment S5 and S6 of human-derived voltage-gated potassium channel 1.3. The amino acid sequence of the peptide fragment is Glu-Ala-Asp-Asp-Pro-Thr-Ser-Gly-Phe-Ser-Ser-Ile-Pro-Asp. An antibody against the peptide fragment prepared by an immunological method can be used as a new, specific inhibitor of human-derived voltage-gated potassium channel 1.3,and the antibody can be used in a study for regulating functions of human-derived voltage-gated potassium channel 1.3. Monoclonal antibodies and vaccines prepared based on the above peptide fragment facilitate the treatment of autoimmune diseases or autoimmune related diseases.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a human-derived voltage-gated potassium channel 1.3 immunogenic peptide and its application. The English full name of this channel is human voltage-gated potassium channel 1.3, hereinafter referred to as hKv1.3. The antibody prepared by using hKv1.3 channel extracellular loop peptide E314, hereinafter referred to as anti-hKv1.3 extracellular loop peptide E314 antibody, can be used as a new and specific inhibitor of hKv1.3 channel. Background technique [0002] Voltage-gated potassium channels are a class of transmembrane proteins that allow potassium ions to pass through cell membranes. They play a role in bioelectric signal transduction of excitable and non-excitable cells such as neurons, muscle cells and lymphocytes. The Kv1.3 channel, one of the members of the voltage-gated potassium channel family, has been confirmed to be abundantly distributed in some specific regions of immun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C07K16/18A61K39/395A61P37/02
Inventor 刘坤杨晓芳杨勇王彦富王朝晖王敏连亦田李晓卫刘金平高翔廖玉华曾秋棠刘琳玲
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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