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Method for examining and analyzing phlegm, and purpose thereof

An analysis method, sputum technology, applied in the field of biomedicine, to achieve good repeatability

Inactive Publication Date: 2011-09-21
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Method for examining and analyzing phlegm, and purpose thereof
  • Method for examining and analyzing phlegm, and purpose thereof
  • Method for examining and analyzing phlegm, and purpose thereof

Examples

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Embodiment 1

[0019] Case collection: According to TCM syndrome differentiation, it was divided into asthma cold phlegm syndrome group, hot phlegm syndrome group, non-cold phlegm heat phlegm syndrome group, and a normal control group. The peripheral blood cell differential count and related inflammatory indicators were measured in each group; the induced sputum technique was used to make the patients cough up deep sputum, and the sputum smear was used for cell differential counting, and the sputum supernatant was collected to detect related inflammatory indicators.

[0020] Main reagents and instruments: 10% sodium chloride (Shanghai Xudong Haipu Pharmaceutical Co., Ltd.); sterile water for injection (Shanghai Fuda Pharmaceutical Co., Ltd.); kit); Swiss staining solution (Amresco); IL-4 kit, INF-γ kit, IL-6 kit, IL-8 kit, IL-10 kit, ECP kit, CRP kit, LTB4 kit Box, IL-5 reagent kit (Biosource imported repackaging); automatic blood analyzer (Abbott CELL-DYN3500R, USA); YS9801 medical ultrason...

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Abstract

The invention belongs to the field of medical science, and relates to a method for examining and analyzing phlegm, and purposes of the method. According to the method, with a phlegm inducting technology, asthma patients with cold- phlegm or heat-phlegm can expectorate deep phlegm. A cytological DC (differential count) is carried out upon the obtained phlegm. Correlated inflammatory indicators in phlegm supernatant and in serums are examined. Reference indexes for cold- phlegm and heat-phlegm microcosmic syndrome differentiation in Chinese medical science of bronchial asthma are provided and the reliability of the indexes is evaluated. As a result, the method provided by the present invention is safe, reliable, and has good repeatability. The method assists in reflecting local inflammation characteristics of airways of patients having asthma, in categorizing bronchial asthma which is a respiratory disease, and in the microcosmic syndrome differentiation of types of syndrome in Chinese medical science. The method can be applied in the researches of pathogenic mechanism, prevention, treatment, and drug screening about bronchial asthma or other respiratory diseases.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a method for detecting and analyzing sputum, in particular to a method for detecting and analyzing changes in cells, cytokines and inflammatory media in sputum. This method is helpful for the classification of respiratory diseases bronchial asthma and the microcosmic differentiation of TCM syndrome types. Background technique [0002] Clinical medical research shows that bronchial asthma is a chronic airway inflammatory disease caused by a variety of inflammatory cells, cytokines and inflammatory mediators, which belongs to the category of asthma in traditional Chinese medicine. Traditional Chinese medicine believes that "subduing phlegm" is the perennial root of asthma. The basic pathological changes are that the phlegm is touched by sensation, the phlegm rises with the qi, and the qi is blocked by the phlegm, and clumps with each other, blocking the airway, causing phlegm to growl like...

Claims

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Application Information

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IPC IPC(8): G01N33/50A61B10/02
Inventor 董竞成曹玉雪崔焱杜懿杰
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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