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Enterococcus faecium and method for producing equol by using Enterococcus faecium and application of Enterococcus faecium

A technology of Enterococcus faecium and enterococcus faecium, which is applied in the field of Enterococcus faecium and its production of equol, and can solve the problems of inability to produce equol and the like

Active Publication Date: 2014-02-05
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these bacteria cannot produce equol in pure culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Enterococcus faecium ( Enterococcus faecium BY1) separation

[0030] Enterococcus faecium ( Enterococcus faecium The preparation method of BY1) is as follows:

[0031] 1. Collection of samples:

[0032] Take 1g of fresh baby feces, put them in 9g normal saline with a mass fraction of 0.85%, store them in a refrigerator at 4°C, and inoculate them within 12h.

[0033] 2. Gradient dilution of samples and selection of equol-producing strains:

[0034] (1) Single colony type separation culture

[0035] Use sterile saline to dilute the pre-prepared samples to 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 , 10 -7 Times, select 10 -5 , 10 -6 , 10 -7 Three gradients were inoculated into the pre-prepared Henggate anaerobic tube containing BHI solid medium, flushed with high-purity nitrogen with a nitrogen blower, and incubated at 37°C for 48 hours.

[0036] (2) Screening of single colonies with the function of producing equol

[0037] Inoculate the single colony obtained from the...

Embodiment 2

[0048] Example 2 Enterococcus faecium ( Enterococcus faecium BY1) Method of producing equol

[0049] The method follows the following steps:

[0050] (1) Enterococcus faecium ( Enterococcus faecium BY1) Strain expansion

[0051] Enterococcus faecium ( Enterococcus faecium BY1) The strain is activated in a 37°C incubator, inoculated into the sterilized BHI medium in a Hengate anaerobic tube at 1 / 20 inoculum, filled with high-purity nitrogen in the nitrogen blowing device, and incubated at 37°C for 48 hours. Obtain expanded culture fermentation broth;

[0052] (2) Bacterial fermentation and metabolism to produce isoflavone composition containing equol

[0053] Prepare isoflavone-BHI medium, in which daidzein content is 14% of the total mass of isoflavones, genistin content is 57% of the total mass of isoflavones, adjust the pH to 6.6, fill the nitrogen blowing device with high-purity nitrogen, and sterilize , Sterile syringe inoculate the expansion fermentation broth in the isoflavo...

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PUM

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Abstract

The invention discloses Enterococcus faecium BY1, a method for producing equol by using the Enterococcus faecium and application of the Enterococcus faecium, and belongs to the field of biochemical industry. The bacteria were collected in China General Microbiological Culture Collection Center on March 28, 2011, and the collection number is CGMCC NO.4707. The Enterococcus faecium has the capacity of transforming a daidzin part in isoflavone into the equol, and can be used for preparing food containing the equol.

Description

Technical field [0001] The present invention is a biochemical industry, which involves a method and application of a feces and enterococcus and its estradhenol. Background technique [0002] The isoflavone substance is a polyphenolic compound, so it is easier to combine with glucose, forming the form of β-glucoside bonds, which is also the main form of isoflavone substances in soy products.However, the coupling isoflavones do not have biological activity, and should not be absorbed by the human body. Under normal circumstances, the form of β-beanosidin in the stomach must first be transformed into the form of β-beanoside to soybeanidin.It is essential to absorb and follow -up. [0003] Equol (Equol) is a derivative of isoflavones, which is the product of the human intestinal bacterial bacteria.At present, one of the metabolic pathways recognized by people can be described as (Juliana, 2003): After the human body consumes soylidoside, etc., the occasional alternate isoflavone is h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P17/06A23L1/29C12R1/46A23L33/00
Inventor 程永强殷丽君刘鹏冯淑环陈明海王盼
Owner CHINA AGRI UNIV