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Method for generating live yeast cell derivative (LYCD) through natural stacking fermentation

A technology of active yeast and natural accumulation, applied in the biological field, can solve the problem of not seeing active yeast cell derivatives

Inactive Publication Date: 2011-10-12
CHINA KWEICHOW MOUTAI DISTILLERY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the production of active yeast cell derivatives by natural solid-state accumulation fermentation

Method used

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  • Method for generating live yeast cell derivative (LYCD) through natural stacking fermentation
  • Method for generating live yeast cell derivative (LYCD) through natural stacking fermentation
  • Method for generating live yeast cell derivative (LYCD) through natural stacking fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for naturally accumulating fermentation to produce active yeast cell derivatives, comprising the following steps:

[0024] (1) The fermentation substrate is mainly crushed and gelatinized sorghum, and other raw materials can be used for solid-state fermentation. Gelatinization, cooling, adding traditional high-temperature Daqu, mixing evenly, stacking, accumulating and fermenting. The amount of koji, the degree of gelatinization of raw materials, and the degree of stacking fermentation are routinely adjusted according to the natural fermentation situation. Fermentation can be repeated multiple times;

[0025] (2) Fermentation time: 3 to 7 days, the fermentation is terminated when the temperature of the fermentation substrate is 52°C and the alcohol concentration is 12% (v / v);

[0026] (3) Centrifuge at 2000g for 20min to collect the bacteria, wash once with sterile water, then add medium to continue culturing for 2h, centrifuge, freeze in liquid nitrogen to b...

Embodiment 2

[0028] A method for naturally accumulating fermentation to produce active yeast cell derivatives, comprising the following steps:

[0029] (1) The fermentation substrate is mainly crushed and gelatinized sorghum, and other raw materials can be used for solid-state fermentation. Gelatinization, cooling, adding traditional high-temperature Daqu, mixing evenly, stacking, accumulating and fermenting. The amount of koji, the degree of gelatinization of raw materials, and the degree of stacking fermentation are routinely adjusted according to the natural fermentation situation. Fermentation can be repeated multiple times;

[0030] (2) Fermentation time: 3 to 7 days, when the temperature of the fermentation substrate is 48°C and the alcohol concentration is 2% (v / v), the fermentation is terminated;

[0031] (3) Centrifuge at 2000g for 20min to collect the bacteria, wash once with sterile water, then add medium to continue culturing for 2h, centrifuge, freeze in liquid nitrogen to b...

Embodiment 3

[0033] A method for naturally accumulating fermentation to produce active yeast cell derivatives, comprising the following steps:

[0034] (1) The fermentation substrate is mainly crushed and gelatinized sorghum, and other raw materials can be used for solid-state fermentation. Gelatinization, cooling, adding traditional high-temperature Daqu, mixing evenly, stacking, accumulating and fermenting. The amount of koji, the degree of gelatinization of raw materials, and the degree of stacking fermentation are routinely adjusted according to the natural fermentation situation. Fermentation can be repeated multiple times;

[0035] (2) Fermentation time: 3 to 7 days, the fermentation is terminated when the temperature of the fermentation substrate is 46°C and the alcohol concentration is 4% (v / v);

[0036] (3) Centrifuge at 2000g for 20min to collect the bacteria, wash once with sterile water, then add medium to continue culturing for 2h, centrifuge, freeze in liquid nitrogen to br...

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Abstract

The invention discloses a method for generating a live yeast cell derivative (LYCD) through natural stacking fermentation. The method is characterized by naturally screening and enriching microorganisms and utilizing the heat generated in the growth and metabolism process of the microorganisms to generate alcohol through fermentation in an anaerobic environment so as to effectively stimulate yeast cells, wherein when the temperature of a fermentation substrate is 46-52 DEG C and the alcohol concentration is 2-12% (v / v), the yeast cells are stimulated to generate the LYCD with higher activity. The LYCD has values in the fields of medicine, health care and beauty.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for producing active yeast cell derivatives. Background technique [0002] Yeast cells have a strong ability to respond to stress. When it is subjected to physical and chemical stress, it will soon synthesize many substances with self-protection and repair properties. These substances have the protection of promoting cell respiration and repair. Sexual substances, called Live Yeast Cell Derivative (LYCD for short), have important medical and cosmetic value. The main active ingredients of LYCD include a large number of stress proteins, peptides and amino acids. [0003] LYCD is a particularly potent and safe active ingredient with a harm-mitigating effect. There have been many cosmetics with yeast and its derivatives as raw materials at home and abroad; LYCD has the effect of stimulating blood vessel formation and accelerating wound healing, and is an extremely effective me...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12R1/645
Inventor 郭坤亮季克良
Owner CHINA KWEICHOW MOUTAI DISTILLERY
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