Fusion protein for resisting chicken coccidiosis, preparation and application thereof

A technology of fusion protein and fusion gene, which is applied in DNA preparation, medical preparations containing active ingredients, hybrid peptides, etc., and can solve problems such as complex life history

Inactive Publication Date: 2013-03-13
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the large genome and complex life history of coccidia, the same coccidia have their own specific antigens and common specific antigens at different developmental stages

Method used

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  • Fusion protein for resisting chicken coccidiosis, preparation and application thereof
  • Fusion protein for resisting chicken coccidiosis, preparation and application thereof
  • Fusion protein for resisting chicken coccidiosis, preparation and application thereof

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Embodiment Construction

[0034] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments, so that those skilled in the art can better understand the present invention and implement it, but the examples given are not intended to limit the present invention.

[0035] 1 Cloning of 3-1E gene

[0036] 1.1 Design and synthesis of primers

[0037] A pair of primers were synthesized by using Primer Premier 5 software with reference to the accession number provided by NCBI,

[0038] Upstream primer P1: 5′-GTT TGT AGT TTC TTT GTA TTT CC-3′;

[0039] Downstream primer P2: 5'-CTG GAG ATC AAT TAG AAG CC-3'.

[0040] Primers were synthesized by Beijing Sanbo Polygala Biotechnology Co., Ltd.

[0041] 1.2 Recovery and purification of coccidia oocysts

[0042] Sporulated oocysts of chicken E.tenella YL strain bred with single oocysts, 1×10 per bird 5 The oocysts were orally infected with 9-day-old coccidian-free male chicks, and the feces were co...

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Abstract

The invention discloses a fusion protein for resisting chicken coccidiosis, a preparation and an application thereof. The invention provides a fusion gene and its expressed fusion protein, wherein the nucleotide sequence of the fusion gene is as shown in SEQ ID NO.1. The invention also constructs a recombinant plasmid containing the above fusion gene. The fusion protein provided by the invention can be used to prepare drugs for preventing chicken coccidiosis.

Description

technical field [0001] The invention relates to a chicken coccidiosis-resistant fusion protein and its preparation and application. Background technique [0002] Coccidia are an important class of parasitic protozoa that seriously endanger the growth and development of poultry. Tyzzeria) and Wenyonella (Wenyonella). Chicken coccidiosis is an intestinal parasitic disease characterized by diarrhea and bloody stools caused by Eimeria parasitizing on the intestinal epithelial cells of chickens, among which Eimeria tenella is more virulent. Its morbidity rate is 50%~70%, mortality rate is 20%~30%, can reach 80% when serious. In addition to morbidity and mortality, other losses such as reduced feed remuneration and reduced carcass quality can also result. It is one of the most frequent, serious and difficult diseases in intensive chicken farming, and one of the most serious economic losses among all animal diseases. The disease is widely distributed and commonly occurs all ove...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/62C12N15/10C12N15/63C07K19/00A61K39/012A61P33/02
Inventor 林青战美娜于三科宋军科李健
Owner NORTHWEST A & F UNIV
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