Synechococcus engineering bacterium capable of improving yield of cellulose, and preparation method and application thereof

A technology of engineering bacteria and cellulose, applied in the biological field, can solve problems such as pollution, unsuitable cultivation and production, and high cost

Active Publication Date: 2012-10-10
PEKING UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the high cost of cultivating Acetobacter xylinum and the pollution of the environment by secondary metabolites and waste produced during the growth process make it unsuitable for large-scale cultivation and production
If you simply use the cellulose it secretes to degrade into ethanol as an energy source, it will require a higher price

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Synechococcus engineering bacterium capable of improving yield of cellulose, and preparation method and application thereof
  • Synechococcus engineering bacterium capable of improving yield of cellulose, and preparation method and application thereof
  • Synechococcus engineering bacterium capable of improving yield of cellulose, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] 1. First, the cellulose synthesis gene cesA (SEQ ID NO: 1) of the cyanobacterium Synechococcus sp. Select a suitable length of DNA fragments at the 5' and 3' ends of the target gene to be knocked out, generally from 400bp to 3Kb. A kanamycin resistance gene is inserted in the middle of the two fragments, and then The three connected fragments are inserted together into a vector that cannot be replicated in cyanobacteria, and the vector is transferred into cyanobacteria, and under the selection of kanamycin, homologous double exchange occurs on the cyanobacteria genome, so that the target gene is replaced by the antibiotic gene. Replaced, so as to achieve the knockout of the target gene. After gene knockout, the original cesA gene deletion, and completely replaced by the kanamycin resistance gene. In addition, the kanamycin resistance gene is inserted into cesA, and The method of not completely knocking out this gene can also make this gene lose its function. In this exp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a synechococcus engineering bacterium and a preparation method and application thereof. The synechococcus engineering bacterium is a cesA gene-deficient Synechococcus sp. PCC 7002 mutant; the plasmid or genome of the mutant is integrated with 7 cellulose synthesis related genes (cmcase, ccp, acsA, acsB, acsC, acsD and bglxA) of Gluconacetobacter xylinum ATCC 53582; and the7 genes are started and expressed by a promoter PcpcBA of a cpcBA gene of Synechococcus sp. PCC 7002 or Synechocystis sp. PCC6803. The cellulose content of the engineering bacterium accounts for about 13% of the dry weight of the cell wall; and simultaneously, the cellulose produced by the synechococcus engineering bacterium has a short growth cycle and does not have the pollution problem of lignin in wood, the extraction and purification processes can be greatly simplified, and the degree of pollution to the environment can also be greatly reduced correspondingly, thus the synechococcus engineering bacterium is an ideal biological engineering bacterium for producing cellulose and is further used as a raw material for producing biological ethanol.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a cyanobacteria engineering bacterium with high cellulose production and its preparation method and application. Background technique [0002] Affected by the energy crisis in recent years, countries all over the world are actively exploring various forms of new energy to replace limited fossil energy (coal, oil, natural gas). As a representative of new energy, bioenergy is playing an increasingly important role. At present, bioenergy mainly includes three categories: bioethanol, biodiesel, and biogas. Ethanol is not only an important chemical raw material, but also a highly efficient and low-pollution alternative energy source for petroleum, so its importance is self-evident. Existing ethanol production platforms mainly rely on the fermentation and decomposition of starch and sugar substances, as well as cellulosic materials such as corn stalks and wheat straw. For ex...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/13C12N15/09C12N15/31C12N15/63C12P19/04C12P7/10C12R1/89
CPCY02E50/16Y02E50/10
Inventor 赵进东赵驰
Owner PEKING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products