A preparation method of silver-loaded modified bacterial cellulose-based composite functional wet dressing

Abstract

The invention relates to a method for preparing a silver-loaded modified bacterial cellulose-based composite functional wet dressing, which comprises the following steps: (1) placing the freeze-dried bacterial cellulose in a nitroxyl/enzyme system solution, adding an oxidizing agent reaction; (2) adding diamine substances to the solution, heating and reacting; (3) then soaking in silver nitrate solution to obtain bacterial cellulose containing selectively loaded silver ions; Obtain bacterial cellulose selectively loaded with nano-silver particles; (4) finally add the compound solution, take it out after standing at room temperature, and after partial drying, packaging, and sterilization, the silver-loaded modified bacterial cellulose-based compound type can be obtained Functional wet dressing. In the method of the invention, the antibacterial nano-silver can be loaded on the C6 hydroxyl group of the bacterial cellulose, and the functional medicine can be compounded. The prepared dressing can treat various skin injuries, wounds, burns and scalds, etc., and has good antibacterial and repairing effects.

Description

technical field

[0001] The invention relates to a method for preparing a silver-loaded modified bacterial cellulose-based composite functional wet dressing. Background technique

[0002] Bacterial cellulose (BC for short) is a kind of cellulose produced by some bacteria. In order to distinguish it from the cellulose produced by plants, it is also called microbial cellulose. Bacterial cellulose is a new biological material with excellent performance, which has been extensively studied in recent years. Although the composition of bacterial cellulose is similar to general cellulose, its physical and chemical properties are unique. Such as: nano-scale ultra-fine network structure; high elastic modulus, tensile strength and wet strength, better mechanical properties than conventional cellulose; good hydrophilicity, because the interior is full of "pores", it has good air permeability and water permeability , water retention capacity; it has good biocompatibility, not only good ...

Examples

Embodiment 1

[0043] (1) the bacterial cellulose produced by Acetobacter xylinum is placed in a freeze dryer and freeze-dried for 24 hours;

[0044] (2) the bacterial cellulose after freeze-drying is immersed in the aqueous solution containing 0.01mol / L 2,2,5,5-tetramethylpiperidine-N-oxyl and 0.01mol / L horseradish peroxidase, The mass-volume concentration of described bacterial cellulose in described aqueous solution is 2g / L, and with the HNO of 1mol / L 3 The solution adjusts the pH of the above aqueous solution to be 2;

[0045] (3) within 1 hour, gradually add dropwise the hypochlorous acid solution of 1mol / L to make the reaction, the amount added dropwise is one-tenth of the volume of the aqueous solution by volume, and the reaction temperature is 100°C;

[0046] (4) The bacterial cellulose treated in the previous step is taken out, washed 5 times with distilled water, and then washed 5 times with ethanol until its pH value is 7, and the selective oxidation of bacterial cellulose is obt...

Embodiment 2

[0054] (1) Place the bacterial cellulose produced by Acetobacter acetogenes in a lyophilizer and freeze-dry for 24 hours;

[0055] (2) The bacterial cellulose after freeze-drying is immersed in the aqueous solution containing 0.1mol / L 4-methyl-TEMPO and 0.1mol / L bean hull peroxidase, the quality of the bacterial cellulose in the aqueous solution -The volume concentration is 5g / L, and the pH value of the above-mentioned aqueous solution is adjusted to 3 with the HCl solution of 5mol / L;

[0056] (3) Gradually drop the hypochlorite solution of 2mol / L in 2 hours to make the reaction, the dripping amount is one-tenth of the volume of the aqueous solution by volume, and the reaction temperature is 20°C;

[0057] (4) The bacterial cellulose treated in the previous step is taken out, washed 3 times with distilled water, and then washed 3 times with ethanol until its pH value is 6, and the selective oxidation of bacterial cellulose is obtained;

[0058] (5) Add the above-mentioned sel...

Embodiment 3

[0065] (1) the bacterial cellulose produced by Acetobacter is placed in a freeze dryer and freeze-dried for 24 hours;

[0066] (2) The bacterial cellulose after freeze-drying is immersed in the aqueous solution containing 0.51mol / L 4-hydroxyl-TEMPO and 0.5mol / L myeloperoxidase, the mass-volume of described bacterial cellulose in described aqueous solution Concentration 10g / L, and use 5mol / L HNO 3 The solution adjusts the pH value of the above aqueous solution to 4, and the reaction temperature is 90°C;

[0067] (3) within 3 hours, the chlorous acid solution of 5mol / L was gradually added dropwise to make the reaction, and the amount of addition was one-tenth of the volume of the aqueous solution by volume;

[0068] (4) The bacterial cellulose treated in the previous step is taken out, washed 5 times with distilled water, and then washed 3 times with ethanol until its pH value is 6, and the selective oxidation of bacterial cellulose is obtained;

[0069] (5) The above-mentione...

technical field

[0001] The invention relates to a method for preparing a silver-loaded modified bacterial cellulose-based composite functional wet dressing. Background technique

[0002] Bacterial cellulose (BC for short) is a kind of cellulose produced by some bacteria. In order to distinguish it from the cellulose produced by plants, it is also called microbial cellulose. Bacterial cellulose is a new biological material with excellent performance, which has been extensively studied in recent years. Although the composition of bacterial cellulose is similar to general cellulose, its physical and chemical properties are unique. Such as: nano-scale ultra-fine network structure; high elastic modulus, tensile strength and wet strength, better mechanical properties than conventional cellulose; good hydrophilicity, because the interior is full of "pores", it has good air permeability and water permeability , water retention capacity; it has good biocompatibility, not only good ...

Examples

Embodiment 1

[0043] (1) the bacterial cellulose produced by Acetobacter xylinum is placed in a freeze dryer and freeze-dried for 24 hours;

[0044] (2) the bacterial cellulose after freeze-drying is immersed in the aqueous solution containing 0.01mol / L 2,2,5,5-tetramethylpiperidine-N-oxyl and 0.01mol / L horseradish peroxidase, The mass-volume concentration of described bacterial cellulose in described aqueous solution is 2g / L, and with the HNO of 1mol / L 3 The solution adjusts the pH of the above aqueous solution to be 2;

[0045] (3) within 1 hour, gradually add dropwise the hypochlorous acid solution of 1mol / L to make the reaction, the amount added dropwise is one-tenth of the volume of the aqueous solution by volume, and the reaction temperature is 100°C;

[0046] (4) The bacterial cellulose treated in the previous step is taken out, washed 5 times with distilled water, and then washed 5 times with ethanol until its pH value is 7, and the selective oxidation of bacterial cellulose is obt...

Embodiment 2

[0054] (1) Place the bacterial cellulose produced by Acetobacter acetogenes in a lyophilizer and freeze-dry for 24 hours;

[0055] (2) The bacterial cellulose after freeze-drying is immersed in the aqueous solution containing 0.1mol / L 4-methyl-TEMPO and 0.1mol / L bean hull peroxidase, the quality of the bacterial cellulose in the aqueous solution -The volume concentration is 5g / L, and the pH value of the above-mentioned aqueous solution is adjusted to 3 with the HCl solution of 5mol / L;

[0056] (3) Gradually drop the hypochlorite solution of 2mol / L in 2 hours to make the reaction, the dripping amount is one-tenth of the volume of the aqueous solution by volume, and the reaction temperature is 20°C;

[0057] (4) The bacterial cellulose treated in the previous step is taken out, washed 3 times with distilled water, and then washed 3 times with ethanol until its pH value is 6, and the selective oxidation of bacterial cellulose is obtained;

[0058] (5) Add the above-mentioned sel...

Embodiment 3

[0065] (1) the bacterial cellulose produced by Acetobacter is placed in a freeze dryer and freeze-dried for 24 hours;

[0066] (2) The bacterial cellulose after freeze-drying is immersed in the aqueous solution containing 0.51mol / L 4-hydroxyl-TEMPO and 0.5mol / L myeloperoxidase, the mass-volume of described bacterial cellulose in described aqueous solution Concentration 10g / L, and use 5mol / L HNO 3 The solution adjusts the pH value of the above aqueous solution to 4, and the reaction temperature is 90°C;

[0067] (3) within 3 hours, the chlorous acid solution of 5mol / L was gradually added dropwise to make the reaction, and the amount of addition was one-tenth of the volume of the aqueous solution by volume;

[0068] (4) The bacterial cellulose treated in the previous step is taken out, washed 5 times with distilled water, and then washed 3 times with ethanol until its pH value is 6, and the selective oxidation of bacterial cellulose is obtained;

[0069] (5) The above-mentione...