processing blood
A blood and blood processing technology, applied in the field of white blood cell separation, can solve the problems of lymphocyte loss, waste of materials, manpower and cost, and time-consuming
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
example 1
[0107] Example 1. Collection of monocytes and enrichment of CD4+ T lymphocytes from peripheral blood
[0108] Bags of peripheral blood were prepared to represent pseudo patients. Collect 4 units of ABO-matched whole blood from healthy donors into ACD-A anticoagulant 1-2 days before use. The blood of the plurality of units was leukocyte-depleted by filtration through a Sepacell leukocyte-reducing filter, and pooled into a 2L blood bag. A leukopak buffy coat was added to bring the white blood cell count to a physiological concentration, and the sham patient bag was kept on a rocking platform at room temperature to ensure a homogeneous cell suspension. A 10 mL sample was taken from the pseudo patient bag and the baseline cell composition was determined by electronic cell counting and automatic differentiation on a Beckman Coulter Act counter, and by using the included CD45-FITC, CD3-PECy7, CD4-APC, CD8-PECy5, CD14 - Flow cytometry of monoclonal antibody panels including PECy7...
example 2
[0127] Example 2. Collection of monocytes from peripheral blood and enrichment of CD8+ cells
[0128] overview
[0129] Figure 12 shown with Figure 7 System 700 is related to improvement of system 1200 . For the sake of brevity, Figure 7 and Figure 12 Like features in the system 1200 retain the same reference numerals (e.g., Figure 7 and Figure 12 Anticoagulant pump 712 in . In addition, unless expressly described below, Figure 12 These like-numbered features in the system 1200 also retain the same configuration. Figure 12 The system 1200 is a blood processing device that involves collection and enrichment (type 2) and contains 3 new bags, 6 new clamps and 2 magnets. Such as Figure 12 As shown, a modification of the standard CellEx process kit was made. A CLINIcell 25 bag 1278 was used instead of the photoactivation chamber. Patients 1200 in Figure 12 Denoted as patient bag #1 1206 in , the patient bag #1 is connected to the return node 756, and the ...
example 3
[0152] Example 3. Collection of monocytes from peripheral blood and enrichment of CD34+ cells
[0153] The collection and enrichment of CD34+ cells can be carried out as described in Examples 1 and 2 using substances that specifically bind to CD34.
[0154] Enrichment of CD34+ cells from collected monocytes
[0155] In one embodiment of the invention, the subject may be mobilized using granulocyte colony stimulating factor (G-CSF). Following completion of standard CellEx monocyte collection, washes were performed with cell enrichment buffer PBS / EDTA (Miltenyi) supplemented with HSA and CD34+ selection beads (Miltenyi) introduced via the needle-free access port of the "collection bag". The monocyte and bead mixture was incubated for 30 minutes while recirculating through the trapping module of the modified CellEx single-use kit and by transferring the cells via a peristaltic pump to a Miltenyi CliniMACS magnet at approximately the manufacturer's recommended flow rate. sys...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com