A kind of preparation method of uncaria total alkali hydrolyzate

A technology of total alkaloids and hydrolysates of Uncaria chinensis is applied in the directions of medical preparations, drug combinations, and pharmaceutical formulations containing active ingredients, which can solve problems such as unfavorable absorption, insolubility of total alkaloids in Uncaria in water, etc. Good water solubility, good desalination effect

Inactive Publication Date: 2011-12-14
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
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Problems solved by technology

Although the two patents have improved the production process of total alkaloids of Uncaria, the total alkaloids of Uncaria produced are insoluble in water, which is not conducive to human body absorption

Method used

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  • A kind of preparation method of uncaria total alkali hydrolyzate
  • A kind of preparation method of uncaria total alkali hydrolyzate
  • A kind of preparation method of uncaria total alkali hydrolyzate

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preparation example Construction

[0033] The preparation of Uncaria total alkaloids of the present invention, step is as follows:

[0034] After drying and crushing the raw material of Uncaria uncaria, extract 1-3 times with 40%-100% ethanol at 4°C-80°C, the ratio of solid to liquid is 1:10-1:50, and the extraction time is 6 h-36h. Filtrate to obtain the total alkaloid extract of Uncaria; concentrate the total alkaloid extract of Uncaria to extract, then add an acid solution to acidify to pH1-pH3, filter, alkalinize the filtrate to pH9-pH10, and then pass the alkalization solution through chloroform Extract 1 to 3 times, let stand to separate layers, collect the chloroform solution, concentrate and dry to obtain the total alkaloid powder of Uncaria. The concentration of the acid solution is 0.1 mol / L to 5.0 mol / L; the acidification temperature is 10°C to 80°C; the acids used in the acid solution include acetic acid, hydrochloric acid, carbonic acid, lactic acid, phosphoric acid, adipic acid, One or a mixture ...

Embodiment 1

[0037] After drying and pulverizing the raw material of Uncaria, extract three times with 70% ethanol by volume ratio, the ratio of solid to liquid is 1:30, the extraction time is 12h, filter to obtain the total alkaloid extract of Uncaria; extract the total alkaloid of Uncaria Concentrate the solution to the extract, add 0.1 mol / L hydrochloric acid to dissolve, acidify to pH2~pH3, then filter, alkalinize the filtrate to pH9~pH10, and extract the alkalized solution three times with chloroform, let it stand for stratification, and collect the chloroform solution, after concentration and spray-drying, the total alkaloids powder of Uncaria can be obtained.

[0038]Weigh 0.1g of Uncaria total alkaloids powder into a container, add 50ml of 0.1mol / L sodium hydroxide solution, control the reaction temperature at about 80°C, and carry out alkaline hydrolysis for 10 minutes. After the reaction, the alkaline solution was collected and acidified with 0.2 mol / L acetic acid solution until ...

Embodiment 2

[0040] Weigh 0.3g of Uncaria total alkaloids powder into a container, then add 30ml of 0.8mol / L sodium carbonate solution, control the reaction temperature to about 70°C, and carry out alkaline hydrolysis for 30min. After the reaction, the alkaline solution was collected and acidified with 2mol / L hydrochloric acid solution until the pH value of the solution was 2.0. After the acidification is finished, filter and collect the acidification solution, pump the acidification solution into a container containing 20g of macroporous resin HPD100, the circulation flow rate is 10BV / h, the adsorption time is 15h, and then pump 80ml of 60% ethanol aqueous solution into the container Desorption is carried out, the circulation flow rate is 12BV / h, the desorption time is 20h, the ethanol aqueous solution is collected, the ethanol aqueous solution is concentrated by rotation and spray-dried to obtain the total alkali hydrolyzate of Uncaria uncaria, and the product yield is 65%. The resulting...

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Abstract

The invention provides a preparation method of a rhomotoxine hydrolysis product. The method comprises the following steps of: performing ester hydrolysis, amide hydrolysis and acidification on rhomotoxine serving as a reaction substrate; and performing resin desalination and a subsequent process to obtain the rhomotoxine hydrolysis product. The preparation method has the advantage that: the obtained rhomotoxine hydrolysis product is water-soluble and is easy for a human body to absorb. On the other hand, the rhomotoxine hydrolysis product can be adsorbed on resin, so that the hydrolysis product can be purified by using resin; and the resin can be recycled without being regenerated, so that the production process is simplified, the production cost is lowered, and the realization of continuous production is facilitated. The method has the advantages of simple reaction process, high efficiency, easiness for controlling, simple process, low energy consumption and high product purity. The rhomotoxine hydrolysis product prepared with the method has characteristic absorption at the position of 224 nanometers, high water solubility and low toxicity, and can well keep the drug action.

Description

technical field [0001] The invention relates to a preparation method of an uncaria total alkali hydrolyzate. Background technique [0002] The Chinese medicine Uncaria is the hooked branch of the Rubiaceae plant Uncaria. Uncaria ( Uncaria rhynchophylla ) There are many varieties, including 15 kinds of Uncaria, Uncaria grandiflora, Uncaria tomentosa, Uncaria sinensis or Uncaria sessileus, which are widely distributed in southwestern my country. It is a medicinal plant that can be cultivated artificially and has abundant resources . Uncaria total alkaloids are deep-processing products of Uncaria, which can be used for high blood pressure, headache, dizziness, cold and convulsions and other diseases. However, with the continuous promotion of the application of total alkaloids of Uncaria, its insoluble characteristics in water limit the application field of total alkaloids of Uncaria to a certain extent. [0003] Chinese invention patent applications 200710098441 and 20071009...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/74A61P9/12A61P1/08A61P25/00A61P25/08A61K135/00
Inventor 甘纯玑甘琴琴杨丹丹谢苗
Owner FUJIAN AGRI & FORESTRY UNIV
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