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A kind of amino plastic substrate and its preparation method and application

A technology for plastic sheets and substrates, which is used in biochemical equipment and methods, determination/inspection of microorganisms, etc.

Active Publication Date: 2011-12-14
CAPITALBIO CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with plastic materials, glass materials are not easy to process and shape, which makes them particularly powerless in the field of biochips such as microfluidics based on various microstructures

Method used

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  • A kind of amino plastic substrate and its preparation method and application
  • A kind of amino plastic substrate and its preparation method and application
  • A kind of amino plastic substrate and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Embodiment 1, prepare two-dimensional amino plastic substrate with aminosilanization method in aqueous solution

[0068] 1. Pretreatment of the film base: Wash the plastic film base with PS deionized water, dry it, and carry out O 3 Treat for 3h or Plasma treatment for 2min under aerobic conditions.

[0069] 2. Aminosilanization: place the plastic sheet base pretreated in step 1 in an aqueous solution of APTS, the concentration of APTS in the solution is 0.01% (volume percentage), and react at 70°C and 120rpm shaking conditions for 30 hours; wash with deionized water and dry to obtain a two-dimensional amino plastic substrate.

[0070] The surface of the plastic sheet is cleaned and modified, and the surface of the plastic sheet is distributed with amino groups by reacting with APTS to obtain an aminoplast substrate (two-dimensional aminoplast substrate).

[0071] The plastic substrate PS was replaced by PC or PMMA, and the above experiment was carried out, and a two-...

Embodiment 2

[0072] Embodiment 2, prepare two-dimensional amino plastic substrate with aminosilanization method in aqueous solution

[0073] 1. Pretreatment of film base:

[0074] With experiment 1 in embodiment 1.

[0075] 2. Aminosilanization: Place the plastic sheet base pretreated in step 1 in an aqueous solution of APTS, the concentration of APTS in the solution is 10% (volume percentage), and react 0.5% at 15°C and 120rpm shaking conditions. hours; wash with deionized water and dry to obtain a two-dimensional amino plastic substrate.

[0076] The plastic substrate PS was replaced by PC or PMMA, and the above experiment was carried out, and a two-dimensional aminoplastic substrate was also obtained.

Embodiment 3

[0077] Embodiment 3, two-dimensional amino plastic substrate is prepared by aminosilanization method in aqueous solution

[0078] 1. Pretreatment of film base:

[0079] With experiment 1 in embodiment 1.

[0080] 2. Amino silanization: place the plastic sheet base pretreated in step 1 in an aqueous solution of APTS, the concentration of APTS in the solution is 0.5% (volume percentage), and react at 35°C and 120rpm shaking conditions for 5 hours; wash with deionized water and dry to obtain a two-dimensional amino plastic substrate.

[0081] The plastic substrate PS was replaced by PC or PMMA, and the above experiment was carried out, and a two-dimensional aminoplastic substrate was also obtained.

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Abstract

The invention discloses an amino plastic substrate and a preparation method and application thereof. The preparation method of a biochip substrate comprises the following step of: connecting an amino group to the surface of a plastic substrate to obtain the substrate with the amino group connected to the surface. Experiments prove that the preparation of a biochip with excellent performance on a plastic material is achieved through the preparation method disclosed by the invention. Because the plastic has strong plasticity and can be conveniently molded, processed and designed into various micro-structures and functional units, the plastic can be applied to microfluidic chips and other biochip fields.

Description

[0001] This application is a divisional application with the application number 200910092146.2, the application date is September 1, 2009, and the invention name is "Aminoplast Substrate and Its Preparation Method and Application". technical field [0002] The invention relates to an amino plastic substrate and its preparation method and application. Background technique [0003] At present, chip technology has become a vital technology for large-scale and high-throughput research in biology. With the development of biochip technology, the material technology and surface chemical technology related to its preparation also need to be continuously improved and improved, which is mainly reflected in the preparation of biochip substrates of different materials. At present, the rigid inorganic material-glass is mainly used for the preparation of biochip substrates, but the substrates made of glass are mainly used for the preparation of passive biochips in which all reactions are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/12C08L69/00C08L25/06C08L33/12C12Q1/68
Inventor 甘五鹏王佳吕品王素珍周玉祥程京
Owner CAPITALBIO CORP
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