Preparation method of Bifidobacterium infantis microcapsules

A technology of Bifidobacterium infantis and microcapsules, which can be used in food preparation, food shaping, food science, etc., can solve problems that have not been applied, and achieve good acid resistance and enteric solubility

Active Publication Date: 2012-01-04
黑龙江省乳品工业技术开发中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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Method used

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  • Preparation method of Bifidobacterium infantis microcapsules
  • Preparation method of Bifidobacterium infantis microcapsules
  • Preparation method of Bifidobacterium infantis microcapsules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Mix the edible gelatin solution and the Bifidobacterium infantis suspension at a speed of 500 rpm, the ratio of the edible gelatin solution and the Bifidobacterium infantis suspension is 1:3, the concentration of the edible gelatin solution is 1.5%, add gum arabic solution, the concentration of gum arabic solution was 1.5%, after stirring at the same speed for 3 min, the ratio of gum arabic solution and edible gelatin solution was 1:1, and the concentration of bifidobacteria suspension was 14.29%. Use 10% acetic acid to adjust the pH of the mixture to 3.8-4.0, continue to stir for 20 min, and put the prepared solution in an ice bath to cool until the temperature is below 10 °C. Use 10% NaOH to adjust the pH of the mixture to 6.0, add transglutaminase at an enzyme concentration of 10 U / g gelatin, continue to stir for 3 min, treat at 10°C for 6 h, wash, filter, etc. The wet bifidobacterium infantis microcapsules can be obtained through the process.

[0022] observed unde...

Embodiment 2

[0024] Mix the edible gelatin solution and the Bifidobacterium infantis suspension at a speed of 400 rpm, the ratio of the edible gelatin solution and the Bifidobacterium infantis suspension is 1:3, the concentration of the edible gelatin solution is 2%, add gum arabic solution, the concentration of gum arabic solution was 2%, and after stirring for 3 min at the same speed, the ratio of gum arabic solution and edible gelatin solution was 1:1, and the concentration of bifidobacteria suspension was 14.29%. . Use 10% acetic acid to adjust the pH of the mixture to 3.8-4.0, continue to stir for 20 min, and put the prepared solution in an ice bath to cool until the temperature is below 10 °C. Use 10% NaOH to adjust the pH of the mixture to 6.5, add transglutaminase at an enzyme concentration of 10 U / g gelatin, continue stirring for 3 min, treat at 15°C for 6 h, wash, filter, etc. The wet bifidobacterium infantis microcapsules can be obtained through the process.

[0025] Take the ...

Embodiment 3

[0027] Take edible gelatin solution and Bifidobacterium infantis suspension and mix at 450rpm, the ratio of edible gelatin solution and Bifidobacterium infantis suspension is 1:3, the concentration of edible gelatin solution is 2%, add gum arabic solution , the concentration of gum arabic solution was 2%, after stirring for 3 min at the same speed, the ratio of gum arabic solution to edible gelatin solution was 1:1, and the concentration of bifidobacteria suspension was 14.29%. . Use 10% acetic acid to adjust the pH of the mixture to 3.8-4.0, continue to stir for 20 min, and put the prepared solution in an ice bath to cool until the temperature is below 10 °C. Use 10% NaOH to adjust the pH of the mixture to 6.0, add transglutaminase at an enzyme concentration of 15 U / g gelatin, continue to stir for 3 min, treat at 15°C for 8 h, wash, filter, etc. The wet bifidobacterium infantis microcapsules can be obtained through the process.

[0028] Take the suspension of newly prepared...

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Abstract

The invention discloses a preparation method of Bifidobacterium infantis microcapsules. The microcapsules are prepared with edible gelatin and gum as microcapsule wall materials and Bifidobacterium infantis as a core material by a water phase separation method. The preparation method uses an enzyme as a cross-linking agent to prepare microcapsules, and the prepared microcapsules have strong gastric acid resistance. The preparation method disclosed by the invention has the advantages of simpleness in process, safety in administration, and on the like. The preparation method improves the utilization ratio of Bifidobacterium infantis, ensures the important roles of Bifidobacterium infantis in regulating microbial balance in infant intestinal tract, promoting infant health growth, and the like, and can effectively promote the application of Bifidobacterium infantis in infant formula milk.

Description

technical field [0001] The invention relates to a preparation method of bifidobacterium microcapsules for infants, especially for formula milk for infants and young children. Background technique [0002] The reduction of Bifidobacterium infantis in the intestinal tract of infants will lead to the growth and reproduction of harmful bacteria, causing intestinal related diseases such as dysentery and constipation. After Bifidobacterium infantis is tested by the extremely acidic conditions of human gastric juice, the number of viable bacteria entering the intestinal tract will be greatly reduced. Therefore, effectively embedding Bifidobacterium infantis and adding it to infant formula has become a research hotspot. Using edible gelatin-gum as the wall material and enzyme as the cross-linking agent to prepare non-toxic and harmless Bifidobacterium infantis microcapsules, which is suitable for the special group of infants and young children, and is conducive to increasing the am...

Claims

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Application Information

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IPC IPC(8): A23L1/29A23P1/04A23L33/00A23P10/30
Inventor 张微满朝新刘宁王贺楠
Owner 黑龙江省乳品工业技术开发中心
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