Manufacturing method of paraffin section of hermatypic coral oocyte

Abstract

The method of the invention comprises the steps of: (1) collecting health coral containing a hermatypic coral oocyte in seafloor; (2) fixing the coral; (3) removing a calcareous skeleton of the hermatypic coral; (4) removing moisture from the texture of the coral; (5) conducting hyalinization to the texture; (6) immersing paraffin into the texture so as to make preparation for sectioning; (7) revising the section; (8) fixing the section on a glass slide; (9) removing the paraffin from the section; (10) carrying out coloration; (11) performing dewatering and section sealing. Considering the characteristic of lime skeleton in hermatypic coral, the method of the invention solves the problem that the lime skeleton of hermatypic coral is unsuitable for making paraffin sections by applying formic acid decalcification method and the like, thus providing accurate technical support for studying hermatypic coral oocyte growth and predicting the ovulation time of hermatypic coral.

Description

technical field [0001] The invention relates to the technical field of making paraffin slices, in particular to a method for making paraffin slices of reef-building coral oocytes. Background technique [0002] Coral reef ecosystems are one of the most productive ecosystems on Earth, providing habitat for a quarter of all marine life and are irreplaceable in marine ecosystems. Due to global climate change, increased marine pollution, transitional marine fishery damage, and large-scale outbreaks of harmful organisms, coral reefs are rapidly degrading worldwide, and the area of ​​coral reefs in the world has decreased dramatically. Therefore, in the study of coral reef ecosystems, the reconstruction and artificial restoration of coral reef ecosystems are research hotspots in this ecological field. [0003] Reef-building corals are the main reef-building organisms and three-dimensional structure builders that form coral reef ecosystems. The proliferation of reef-building cora...

AI Technical Summary

Problems solved by technology

This method will be very difficult in the whole underwater operation process when the waves are rough and the current is fast.

The experience of underwater operators is different, and there will be various differences in the judgment of the oocyte development degree of reef-building corals, which will eventually lead to inaccurate prediction of ovulation time of reef-building corals

At the same time, due to uncertain factors such as underwater brightness and seawater refraction, it is easy for underwater operators to judge the color of reef-building coral oocytes, and ultimately affect the prediction of reef-building coral ovulation time.

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