Botryococcus braunii magnetic separation method
A technology for botrytis braziliana and magnetic separation, which is applied in the direction of single-cell algae, oxides of ferrous iron, nanotechnology, etc., can solve the problem of low yield and quality of botrytis brachyphyllum cells, and does not consider the coupling of oil extraction and separation medium recovery , Particles cannot be recycled and recycled, etc., to achieve the effect of easy amplification, lower production costs, and realization of regeneration and recycling
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Embodiment 1
[0055] Using the high-efficiency recovery method of magnetic nanoparticles for the magnetic separation of Staphylococcus branici of the present invention, Fe in the magnetic separation of Staphylococcal 3 o 4 The recovery of nanoparticles, the steps are as follows:
[0056] (1) Fe obtained by preparing 3 o 4 The nanoparticles are dispersed in deionized water to form a stable suspension, prepared into a magnetic separation medium with a concentration of 1% (w / v), and then put into a refrigerator for further storage.
[0057] (2) put the botrytis brannica culture solution obtained by cultivating into a stirred reactor, add a magnetic separation medium at a rotating speed of 200rpm, the consumption of the magnetic separation medium is 0.02% (w / v), and the stirring time is 1.5 minutes; After the separation, the supernatant was removed, and the separated botrytis brachyphytum cells and the polymer of the magnetic medium were collected, and the recovery rate of the botrytis brach...
Embodiment 2
[0063] Using the high-efficiency recovery method of magnetic nanoparticles for the magnetic separation of Staphylococcus branici of the present invention, Fe in the magnetic separation of Staphylococcal 3 o 4 The recovery of nanoparticles, the steps are as follows:
[0064] (1) Fe obtained by preparing 3 o 4 The nanoparticles are dispersed in deionized water to form a stable suspension, prepared into a magnetic separation medium with a concentration of 0.5% (w / v), and then put into a refrigerator for storage.
[0065](2) Put the botrytis culture solution obtained by cultivation into a stirring reactor, add a magnetic separation medium at a rotating speed of 100rpm, the consumption of the magnetic separation medium is 0.01% (w / v), and the stirring time is 2 minutes; Finally, the supernatant was removed, and the isolated botrytis brachyphytum cells and the polymer of the magnetic medium were collected, and the botrytis recovery rate reached 99.5%;
[0066] (3) At room temper...
Embodiment 3
[0071] Using the high-efficiency recovery method of magnetic nanoparticles for the magnetic separation of Staphylococcus branici of the present invention, Fe in the magnetic separation of Staphylococcal 3 o 4 The recovery of nanoparticles, the steps are as follows:
[0072] (1) Fe obtained by preparing 3 o 4 Nanoparticles are dispersed in deionized water to form a stable suspension, prepared into a magnetic separation medium with a concentration of 2% (w / v), and then put into a refrigerator for further storage.
[0073] (2) Put the botrytis culture solution obtained by cultivation into a stirred reactor, add a magnetic separation medium at a rotating speed of 300rpm, the consumption of the magnetic separation medium is 0.03% (w / v), and the stirring time is 1 minute; Finally, the supernatant was removed, and the isolated botrytis brachyphytum cells and the polymer of the magnetic medium were collected, and the botrytis recovery rate reached 99.3%;
[0074] (3) At room tempe...
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