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Fermented intestine-strengthening cheese/powder production method and its applied lactobacillus casei

A technology of Lactobacillus casei and its manufacturing method, which is applied in the field of manufacturing fermented intestine-healthy cheese/powder, can solve the problems of low live bacteria content, reduced milk protein antigenicity, and low lower limit value, so as to achieve a refreshing and non-greasy taste, reduce Allergic antigen protein content, effect of caloric reduction

Inactive Publication Date: 2012-02-01
泰州市科星生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, cheese / powder is mainly fermented by a single strain or several strains of Lactobacillus, and there is no product that uses Lactobacillus and Bifidobacteria for fermentation at the same time. The content of live bacteria in these cheese / powders is too low. In addition, the lower limit set by the national standard for milk beverages is relatively low, generally below 105 per milliliter. The functionality of the strains used is to improve the intestinal flora and enhance immunity inherent in general probiotics. As a major health claim, there is no strain that reduces the antigenicity of milk protein

Method used

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  • Fermented intestine-strengthening cheese/powder production method and its applied lactobacillus casei
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  • Fermented intestine-strengthening cheese/powder production method and its applied lactobacillus casei

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Embodiment Construction

[0010] The present invention is a kind of Lactobacillus casei, the classification name is Lactobacillus casei, and the biological material (strain) of reference: JY68, this strain is preserved in the general microorganism center of China Microbial Strain Preservation Management Committee, the address is: Chaoyang, Beijing No. 3, No. 1, Beichen West Road, District, Institute of Microbiology, Chinese Academy of Sciences, postal code: 100101, its preservation number is CGMCC No.3567, and the preservation date is January 12, 2010. The result is survival. The antigenic value of the fermented product of Lactobacillus caseiJY68 can be reduced to 1 / 1000 of that before fermentation, which is obtained through the following experiments:

[0011] 1. Protease activity and peptidase activity test of lactic acid bacteria strains: first, 4 strains were purchased from Japan: lactobacillus casei subsp.casei L-14, Streptococcus lactis subsp.cremoris H-61, Streptococcus lactis subsp.lactis 527; St...

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Abstract

The invention discloses lactobacillus casei Lc JY68, the collection number of which is CGMCC No. 3567. The invention also discloses a fermented intestine-strengthening cheese / powder production method, which comprises the following steps of: 1, preparing the lactobacillus casei Lc JY68 and a bifidobacterium breve BbQQ12 stain; 2, inoculating the lactobacillus casei Lc JY68 strain nutrient solution individually or with lactobacillus casei subsp casei into a MRS medium for fermentation at constant temperature, inoculating the bifidobacterium breve BbQQ12 stain into a TOS medium for fermentation; 3, cooling the two fermented strains obtained from Step 2, followed by centrifugation, and collecting thalline; 4, adding a freeze-drying protective agent into the thalline, pre-freezing at low temperature, freeze-drying to obtain the fermented intestine-strengthening cheese / powder, and finally packaging.

Description

technical field [0001] The invention relates to a method for manufacturing fermented intestine-healthy cheese / powder and the Lactobacillus casei used therein. Background technique [0002] At present, cheese / powder is mainly fermented by a single strain or several strains of Lactobacillus, and there is no product that uses Lactobacillus and Bifidobacteria for fermentation at the same time. The content of live bacteria in these cheese / powders is too low. In addition, the lower limit set by the national standard for milk beverages is relatively low, generally below 105 per milliliter. The functionality of the strains used is to improve the intestinal flora and enhance immunity inherent in general probiotics. As a major health claim, there is no strain that reduces the antigenicity of milk protein. Contents of the invention [0003] The invention provides a method for manufacturing fermented intestine-healthy cheese / powder and the Lactobacillus casei used therein. It can not...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/127C12R1/245
Inventor 赵景阳
Owner 泰州市科星生物技术有限公司