Method for separating hemopoietic stem cells through cord blood separation double bag

A technology of hematopoietic stem cells and umbilical cord blood, which is applied in the field of separating stem cells, can solve problems such as being easily contaminated, and achieve the effects of saving operation time, high safety performance, and safe and effective concentration

Active Publication Date: 2012-02-01
江苏省北科生物科技有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the shortcomings of the transfer of hematopoietic stem cells in the prior art, the open operation of separation is easy to be polluted, etc., the present invention provides a method for separating hematopoietic stem cells in a double bag for cord blood separation. The method is airtight, safe and effective, and has no hidden danger of pollution

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  • Method for separating hemopoietic stem cells through cord blood separation double bag
  • Method for separating hemopoietic stem cells through cord blood separation double bag
  • Method for separating hemopoietic stem cells through cord blood separation double bag

Examples

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Embodiment 1

[0030] A method for separating hematopoietic stem cells with two bags of umbilical cord blood separation, comprising a 200ml main bag 1 and a 150ml transfer bag 2, the main bag is connected with a feeding tube 7 and a transfer connecting tube 3, and the feeding tube is provided with a feeding control The valve 10 and the 0.22um filter 11 are connected with the parallel inlet blood vessel 6 and the transfer tube 5 on the transfer tube, the blood inlet control valve 9 and the blood filter 13 are arranged on the inlet blood vessel, and the transfer tube 5 is connected with the transfer bag 2, and The transfer pipe 5 is also provided with a transfer control valve 8, the transfer connecting pipe 3 is also provided with a sampling port 12, and the main bag is also provided with a cell outlet 4 externally connected to a cell freezing bag;

[0031] The method of isolating hematopoietic stem cells comprises the steps of:

[0032] (a) After mixing the blood in the umbilical cord blood c...

Embodiment 2

[0045] The method of isolating hematopoietic stem cells comprises the steps of:

[0046] (a) After mixing the blood in the umbilical cord blood collection bag, centrifuge at a speed of 2000 rpm for 30 minutes;

[0047] (b) Transfer the upper layer of blood to the main bag through the inlet blood vessel with the blood filter through the blood separation clamp, and the remaining 20ml of red blood cells will not be transferred and discarded;

[0048] (c) Add 1 / 5 volume of hydroxyethyl starch HES from the feeding tube with a 0.22um filter to the main bag, and close the transfer control valve after transfer;

[0049] (d) Mix the blood components of the main bag with hydroxyethyl starch HES, put the cord blood separation double bag into a centrifuge as a whole, and centrifuge at 1500 rpm for 15 minutes with the main bag interface facing up;

[0050] (e) Open the transfer control valve, and transfer the 2 / 3 plasma that was placed on the upper layer of the main bag after centrifugation...

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Abstract

The invention relates to a method for separating hemopoietic stem cells through a cord blood separation double bag. According to the method provided by the invention, red blood cells and blood plasma are separated and transferred to a transfer bag step by step by adopting a main bag and the transfer bag, and a freezing protection liquid is added, the cells are transferred to a cell freezing bag and are finally frozen and stored. The method realizes the separation of the hemopoietic stem cells by adopting a centrifugal method, the structure of the adopted transfer bag for separation is simple and practical, the overall separation process is simple and convenient, the separation is totally enclosed, the pollution is reduced, the storage volume of the hemopoietic stem cells can be safely and effectively concentrated, and the recovery rate of the hemopoietic stem cells can be improved as much as possible.

Description

technical field [0001] The invention relates to a method for isolating stem cells, in particular to a method for isolating hematopoietic stem cells by using a double bag for separating umbilical cord blood. Background technique [0002] Stem cells are a kind of pluripotent cells with self-replication ability, which can differentiate into various functional cells under certain conditions. With the deepening of stem cell research, the scope of its clinical application is also expanding. Hematopoietic stem cells are the earliest stem cells used clinically, and are the only source of various blood cells in the body. They mainly exist in bone marrow, peripheral blood, and umbilical cord blood. Transplantation of hematopoietic stem cells is an effective method for the treatment of blood system diseases, immune system diseases, congenital genetic diseases, and multiple and metastatic malignant tumor diseases. [0003] Umbilical cord blood has become an important source of hema...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0789
Inventor 李俊陈林李晓鸣宋云庆杨兵
Owner 江苏省北科生物科技有限公司
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