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Enzymolysis method of starchy material and preparation method of citric acid

A starchy raw material, enzymatic hydrolysis technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low yield of citric acid and high production cost of citric acid finished products, reduce starch aging, reduce Production cost, effect of controlling the degree of liquefaction

Inactive Publication Date: 2013-09-25
COFCO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the residual starch content in the enzymolysis product obtained by the existing enzymatic hydrolysis starchy raw material process is relatively high, therefore, the yield of citric acid is low, and the production cost of the finished product of citric acid is relatively high.

Method used

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  • Enzymolysis method of starchy material and preparation method of citric acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] This example is used to illustrate the enzymatic hydrolysis method of corn raw material and the method for preparing citric acid of the present invention.

[0048] (1) Grinding of corn raw materials

[0049] 100 parts by weight of corn (with a moisture content of 14% by weight) were pulverized to obtain a pulverized product with an average particle diameter of 400 microns, and the pulverized product was mixed with 300 parts by weight of water to obtain a starch slurry.

[0050] Determine the total sugar content in the mixed slurry according to the total sugar direct titration method of GBT 15038-2006, wherein the weight of the total sugar is 1.11 times the weight of the starch in the crushed product, therefore, the total sugar content can be determined by the national standard method According to the formula: starch content=total sugar / 1.11, the calculated starch content in the pulverized product is 63 parts by weight / 100 parts by weight corn.

[0051] (2) Enzymolysis ...

Embodiment 2

[0069] This example is used to illustrate the enzymatic hydrolysis method of corn raw material and the method for preparing citric acid of the present invention.

[0070] (1) Grinding of corn raw materials

[0071] 100 parts by weight of the same corn raw material as in Example 1 were pulverized to obtain a pulverized product with an average particle diameter of 600 microns, and the pulverized product was mixed with 300 parts by weight of water to obtain a starch slurry.

[0072] (3) Enzymolysis

[0073] At 60°C, the starch slurry obtained in step (1) is mixed with the enzyme of 35% by weight of the total weight of the enzyme to obtain a mixture, and the mixture is sprayed into contact with steam at 255°C in an injector (steam and mixture The weight ratio is 0.06: 1), the contact time is 1 second, so that the temperature of the mixture after contacting with steam is 90°C, the pH value is adjusted to 5, and maintained at this temperature for 80 minutes;

[0074] The above-men...

Embodiment 3

[0085] This example is used to illustrate the enzymatic hydrolysis method of corn raw material and the method for preparing citric acid of the present invention.

[0086] (1) Grinding of corn raw materials

[0087] 100 parts by weight of the same corn raw material as in Example 1 were pulverized to obtain a pulverized product with an average particle diameter of 300 microns, and the pulverized product was mixed with 300 parts by weight of water to obtain a starch slurry.

[0088] (2) Enzymolysis

[0089] At 55°C, the starch slurry obtained in step (1) is mixed with 25% by weight of the enzyme of the total weight of the enzyme to obtain a mixture, and the mixture is sprayed into contact with steam at 240°C in an injector (steam and mixture The weight ratio is 0.05: 1), the contact time is 1.5 seconds, so that the temperature of the mixture after contacting with steam is 85°C, the pH value is adjusted to 5, and maintained at this temperature for 70 minutes;

[0090] The above-...

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Abstract

The invention relates to an enzymolysis method of a starchy material. The method comprises the steps of: (1) mixing a starch slurry obtained from a starchy material with part of enzymes so as to obtain a mixture, contacting the mixture with steam so as to make the temperature of the steam contacted mixture ranging from 75 to 95DEG C, under which the mixture is maintained for 30-90min; (2) contacting the steam contacted mixture in step (1) with steam again so as to make the temperature of the steam re-contacted mixture ranging from 100-120DEG C, under which the mixture is maintained for 1-10min; (3) cooling the steam re-contacted mixture to a temperature of 80-90DEG C, and mixing the cooled mixture with the residual enzymes for enzymolysis. The invention also provides a preparation method of citric acid. The methods provided in the invention provides a solution to the problem of high content of residual starch in an enzymolysis product (liquefied fluid), and can effectively enhance citric acid yield and reduce grain consumption, thus lowering the production cost of citric acid.

Description

technical field [0001] The invention relates to an enzymatic hydrolysis method of starchy raw materials and a preparation method of citric acid. Background technique [0002] Citric acid is an organic acid widely used in industries such as beverage, food and medicine. my country is a major producer of citric acid, with more than 20 production plants in the country, with an annual output of more than 800,000 tons. At present, citric acid is mainly prepared by fermentation. For example, it is generally necessary to pulverize the starchy raw material first, mix the pulverized product with water to obtain a starch slurry, and mix the starch slurry with an enzyme for enzymolysis to obtain an enzymatic hydrolysis product (liquefied liquid ), and Aspergillus niger was inoculated into the fermentation broth containing the enzymatic hydrolysis product and fermented to produce citric acid. However, the residual starch content in the enzymolyzed product obtained by the existing proce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P7/48C12R1/685
Inventor 周永生杨冬周友超章辉平
Owner COFCO BIOTECHNOLOGY CO LTD
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