Primers, probe and kit for detecting candida dubliniensis

Abstract

The invention provides a kit for detecting candida dubliniensis. The kit comprises specific primers, a probe and other reagents required for detection. The specific primers comprise specific primers F and R, wherein the primer F has a nucleotide sequence shown as SEQ ID No. 1 in a sequence table, the primer R has a nucleotide sequence shown as SEQ ID No. 2 in the sequence table, and the probe has the nucleotide sequence shown as SEQ ID No. 3 in the sequence table. The kit provided by the invention has the advantages of strong specificity, high detection sensitivity, high speed and the like, and is very suitable for being widely used clinically.

Description

technical field [0001] The invention relates to the field of detection of Candida, in particular to a primer, a probe and a kit for detecting Candida Dublin. Background technique [0002] Candida dubliniensis (Candida dubliniensis, also known as Candida dunili), belonging to the subphylum Bacillus, class Cryptococcus, order Cryptococcus, Candida genus, is a germ tube and Firmicutes positive chance Pathogenic yeast is a new strain that was recognized in July 1995. Candida Dublin was isolated from HIV-infected patients with extremely low immunity, and the infection site was mostly in the oropharynx. With the increasing research on Candida Dublin, Candida Dublin has also been found in other parts of the infected person, such as vagina, lungs, blood, heart, etc. [0003] In recent years, due to the widespread use of broad-spectrum antibiotics, the development of cancer chemotherapy, organ transplantation, and the increase in immunocompromised patients, especially AIDS patients...

AI Technical Summary

Problems solved by technology

Its advantages are simple, fast and cheap, but it can only distinguish Candida from other cocci, and cannot distinguish Candida Dublin from Candida albicans

[0007] 2. Culture method: It is an important basis for clinically determining the diagnosis and treatment of Candida at present, but clinically different Candida grows on Sabouraud medium and all present white colonies, so it is necessary to distinguish Candida Dublin and Candida albicans, It is also necessary to do the thick-walled spore test and the germ tube test of Candida. A large number of experiments have proved that both Candida dublin and Candida albicans can form thick-walled spores within 48 hours, and Candida albicans thick-walled spores generally appear as a single Terminal, Candida dublinis has a large number of thick-walled spores, which are generally double-terminal or clustered, but these characteristics do not exist stably, so it is still impossible to accurately and effectively distinguish Candida dublini from Candida albicans

Most of these rapid identification systems are applied to the identification of clinical yeasts, and the types of yeasts that can be identified are few. They are only suitable for basic research and are not conducive to the promotion and use of small and medium-sized hospitals.

[0011] (3) Immunological determination: latex agglutination reaction, radioimmunoassay and enzyme-linked immunosorbent assay can also be used as auxiliary detection means for Candida, but their sensitivity and specificity are poor, and Candida Dublin and Candida cannot be successfully separated candida albicans

This method currently plays a dominant role in the monitoring, prevention and control of daily nosocomial infection, but it has many shortcomings such as low resolution, many influencing factors, and poor repeatability.

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