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Rice syrup extractive and application thereof

A rice syrup and extract technology, applied in sugar derivatives, measuring devices, instruments, etc., can solve problems such as difficult detection of honey, and achieve the effect of simple operation, high accuracy rate, and true and reliable detection results

Inactive Publication Date: 2012-03-21
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the defect that existing rice syrup adulterated honey is difficult to detect, the purpose of the present invention is to provide a kind of rice syrup extract, and provide a kind of detection method of rice syrup adulterated honey according to this extract

Method used

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  • Rice syrup extractive and application thereof
  • Rice syrup extractive and application thereof
  • Rice syrup extractive and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Weigh 1000g of rice syrup, add 500ml of water to dissolve, and purify the syrup solution in batches through OASIS HLB (6ml, 500mg) column, each time 30ml of OASIS HLB sample solution, after the sample solution flows out, wash with 3ml of 20% acetonitrile aqueous solution All the eluents were combined, concentrated at 60°C to a viscous liquid, and then dissolved in 50ml of water.

[0029] Adopt preparative liquid chromatography, chromatographic column Zorbax SB-C18, 21.2 * 250mm, mobile phase is 98% water+2% acetonitrile (V / V), flow velocity 15ml / min, injection volume 10ml, detection wavelength 280nm, collect The component after 7.5-10.5 minutes is the target substance. Freeze-dry the collected liquid to obtain a freeze-dried product. According to the concentration of general raw materials, 1 kg of rice syrup can obtain about 4 mg of this substance. The infrared and ultraviolet spectra of the substance are shown in Figure 1a and 1b shown.

[0030] 1 H NMR (400MHz, DM...

Embodiment 2

[0033] Sample source:

[0034] 150 real honey samples from the Bee Product Quality Supervision, Inspection and Testing Center of the Ministry of Agriculture, all of which come from contracted bee factories, and are identified by electronic traceability technology, which can prove that these samples are pure honey. 6 parts of suspected rice syrup adulterated honey: Take 6 parts of real honey, and then take 4 parts of it to be mixed with rice syrup to form honey containing 5% rice syrup by weight, and use the same container to make it indistinguishable in appearance. 32 parts of rice syrup samples come from different provinces and cities of rice syrup production plants, arbitrarily get 1 part of rice syrup in 32 parts of rice syrup samples, and get a small amount of real honey samples to be configured into honey containing 5% rice syrup by weight.

[0035] Analyze with liquid chromatography, use rice syrup extract (formula I) as the target substance to identify these samples, de...

Embodiment 3

[0047] The 21 raw honey samples submitted by a honey company were required to be tested for rice syrup. Using the method of the present invention, using the rice syrup extract POLY-S as the target substance, tandem mass spectrometry is used to confirm whether the samples to be tested are mixed with rice syrup.

[0048] (1) Sample processing:

[0049] Weigh 10g sample, dissolve it with 15mL deionized water, transfer the solution to OASISHLB (200mg, 6mL) small column, activate the small column extraction with 5ml methanol and 10ml water in advance, when the sample liquid flows out, use 3ml 20% acetonitrile Aqueous solution was used for elution, and the eluate was filtered through a filter membrane, and the filtrate was to be determined.

[0050] (2) Liquid chromatography tandem mass spectrometry conditions:

[0051] Agilent 6460 tandem mass spectrometer, column Agilent AQ C18, 2.1×100, 3.5um; column temperature: 30°C; mobile phase: acetonitrile + water (volume ratio), gradient...

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Abstract

The invention relates to a rice syrup extractive, which has the structure as shown in formula I. The invention also relates to the application of the extractive in rice syrup adulterated honey detection. The invention further relates to a detection method for rice syrup adulterated honey, which takes the rice syrup extractive as a target material, and the honey which is detected with the target material is the rice syrup adulterated honey. The detection method takes the rice syrup extractive as an identification material, only a small mount of sample can detect the material, and the detectionaccuracy rate is high. The method is simple to carry out, and has wide application value.

Description

technical field [0001] The invention relates to the field of analysis and detection of honey, in particular to a rice syrup extract and its application, and a detection method for rice syrup adulterated honey using the extract as a target substance. Background technique [0002] Honey is a natural sweet substance that bees collect from the nectar, secretion or honeydew of plants, combine with their own secretions, and fully brew. Because honey has important nutritional value and health care function, it has been loved by people for a long time. Honey is an extremely easy food to be adulterated. Due to the movement of interests, in recent years, some self-employed and manufacturers have mixed sugar, invert sugar, fructose, glucose, glucose syrup, fructose, etc. into honey to make adulterated honey. Some manufacturers even use glucose, fructose syrup, etc. Honey, honey essence and other alternative raw materials are used to make honey, and put on the market in the form of nat...

Claims

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Application Information

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IPC IPC(8): C07H17/04G01N30/88G01N27/68
Inventor 薛晓锋吴黎明李熠陈本晶赵静陈兰珍周金慧张金振陈芳
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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