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Rotary cell culture device

A cell culture and rotating device technology, applied in tissue cell/virus culture devices, biochemical instruments, biochemical equipment and methods, etc., can solve the problems of uneven distribution of nutrient elements, difficulty in large-scale cultivation, and restriction of cell proliferation, etc. Achieve the effect of simple structure, low cost and large cell density

Active Publication Date: 2013-05-29
BEIJING DOING TIMES BIOMEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the static culture of cells, during the cell growth process, due to the static culture medium and uneven distribution of nutrients, the proliferation of cells is limited, so the cell density is not high, and it is difficult to cultivate on a large scale

Method used

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  • Rotary cell culture device
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Embodiment Construction

[0025] In order to further illustrate the structure and function of the present invention, the present invention will be described in detail below in conjunction with the accompanying drawings and preferred embodiments, but it should be understood that the protection scope of the present invention is not limited by specific embodiments.

[0026] figure 1 It is a schematic bottom view of the cell culture device of the present invention. As shown in the figure, the culture device includes a support 2 , a driving device, a roller 1 and a culture bag 12 .

[0027] The bracket 2 includes a rectangular bottom plate 3, the above-mentioned driving device is arranged on the left side of the bottom plate 3, and on the right side of the bottom plate 3, a set of fulcrum wheels is provided, and the set of fulcrum wheels includes two rollers 11 for supporting drum 1;

[0028] The driving device is arranged on the left side of the base plate 3, and includes a motor 6, an electric control de...

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PUM

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Abstract

The invention discloses a rotary cell culture device. The device is characterized by comprising a bracket, a drive device, a rotatable device and a culture bag, wherein the drive device is arranged on the bracket and used for driving the rotatable device to rotate; and the culture bag is of a cylindrical structure formed by a hydrophobic air-permeable material, and is detachably connected to the rotatable device and can coaxially rotates with the rotatable device. The rotary cell culture device can be used for large-scale culture of suspension cells, can obtain larger cell density on the premise of not influencing cell activity, and rolls mildly in the culturing process to prevent a culture medium from generating foam.

Description

technical field [0001] The invention relates to a cell culture device, in particular to a rotary cell culture device for dynamic cell culture. Background technique [0002] At present, cell culture is divided into dynamic culture and static culture. Among them, dynamic cell culture systems include stirring type, roller bottle type, and shaker type. Agitated culture is mainly used for suspension cell culture, or microcarriers are added to the culture medium to allow adherent cells to grow on the microcarriers to increase the surface area of ​​cell growth. However, when cells are cultured under agitation, the shear force formed by the liquid flow easily damage cells. Roller bottle culture is mainly used for the culture of adherent cells. The advantage of using roller bottle culture is that it can increase the surface area for cell attachment, and at the same time, gentle rotation is conducive to cell growth. When the culture bottle rotates, the cells leave the culture soluti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/02
CPCC12M23/08C12M23/14C12M23/24C12M23/48C12M27/10C12M41/02
Inventor 李刚毅盖丽云李波
Owner BEIJING DOING TIMES BIOMEDICAL TECH