Method for changing content of chlorophyll in plant

A technology of chlorophyll content and chlorophyll, which is applied in the field of changing chlorophyll content in plants, can solve problems such as the complex regulation network of chlorophyll synthesis and degradation, and achieve the effect of broad application prospects

Active Publication Date: 2012-04-11
FUJIAN SANAN SINO SCI PHOTOBIOTECH CO LTD
View PDF1 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the synthesis of chlorophyll is regulated by internal and environmental factors, and this regulation is controlled by nuclear genes and (or) plastid genes. Therefore, there is a complex regulatory network for the synthesis and degradation of chlorophyll.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for changing content of chlorophyll in plant
  • Method for changing content of chlorophyll in plant
  • Method for changing content of chlorophyll in plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, the method for changing chlorophyll content in the plant

[0039] 1. Construction and verification of interference vectors

[0040] The vector pKANNIBAL has been disclosed in literature (Chris Helliwell and Peter Waterhouse, Methods, 2003, 30: 289-295), and can be obtained from the Institute of Botany, Chinese Academy of Sciences.

[0041] The binary vector pART27 has been disclosed in literature (Andrew P Gleave, Plant Molecular Biology, 1992, 20: 1203-1207), and can be obtained from the Institute of Botany, Chinese Academy of Sciences.

[0042] Agrobacterium strain GV3101 has been disclosed in literature (Steven J Clough and Andrew F Bent, Plant Journal, 1998, 16: 735-743), and the public can obtain it from the Institute of Botany, Chinese Academy of Sciences.

[0043] During the construction of the plant antisense suppression vector, the PCR amplified fragment can also be obtained according to the following method: artificially synthesize the DNA fragm...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for changing the content of chlorophyll in a plant. The method comprises the following step of making the FHY3 protein encoding gene of a starting plant to lose function to obtain a target plant, wherein the content of chlorophyll in the target plant is lower than that of the starting plant and / or photochemical efficiency of a photosystem II of the target plant is lower than that of the starting plant. According to the invention, a transgenic plant is obtained by using RNAi (ribonucleic acid interference) to inhibit the FHY3 gene. The content of chlorophyll in leaves of the transgenic plant is lowered, thus the color of the leaves is light and the photochemical efficiency of photosynthesis is lowered. The method disclosed by the invention has a broad application prospect in the field of plant genetic breeding.

Description

technical field [0001] The present invention relates to a method for changing the chlorophyll content in plants. Background technique [0002] Photosynthesis is the process of using solar energy on the largest scale on earth, which provides organic matter, energy and oxygen for almost all life activities. About 220 billion tons of organic matter are synthesized through photosynthesis on the earth every year, which is equivalent to 10 times the annual energy consumption required by human beings. 90%-95% of plant dry matter comes from the products of photosynthesis, and photosynthesis is the material basis for crop yield formation. Photosynthesis is mainly carried out in the chloroplast of plant leaves, which requires the participation of a series of pigments and protein complexes. Chlorophyll is the main pigment that absorbs light energy. The reduction or absence of chlorophyll will make the color of leaves lighter and directly affect the efficiency and function of photosyn...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N15/113C12N15/29C12N15/11C07K14/415A01H5/00
Inventor 林荣呈唐为江景艳军
Owner FUJIAN SANAN SINO SCI PHOTOBIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap