Callosobruchus maculates standard sample and preparation method thereof
A technology of the four-striped bean weed and standard samples, which is applied in the field of plant quarantine, can solve the problems of no insect standard sample research, identification results that cannot be traced, inedible, and used for planting, etc., to eliminate the difference in results and ensure accuracy and reliability. The effect of sex and good mobility
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Embodiment 1
[0041] The preparation method of the four-striped bean elephant standard sample, the concrete steps are as follows:
[0042] The first step, the source of the standard sample
[0043] The source of the standard sample of Elephus sinensis was intercepted from the imported goods; the intercepted Elephus four-striped goods were identified by experts and raised as parents.
[0044]No matter which country or region they come from, the intercepted imported weeds should be kept in isolation. After one generation, observe whether the traits of the progeny are separated, and then select the parent as a standard sample. For the intercepted insects, it is necessary to pay attention to whether they are alive in order to prepare for the next step of propagation. Keep a record of intercepted insects, in particular clearly labeling the country from which the insect came from. According to the corresponding growth conditions of different insects, select the growth conditions such as tempera...
Embodiment 2
[0061] The four-lined bean elephant standard sample that above-mentioned embodiment makes carries out homogeneity and stability test:
[0062] (1) Uniformity test
[0063] The uniformity test is completed in two steps, one is the "in-bottle" uniformity test, and the other is the "between-bottle" uniformity test.
[0064] When designing the "in-bottle" uniformity inspection scheme, the main consideration is to minimize the differences among individual insects. Due to the diversity of organisms, it is inevitable that there will be some differences between each individual of the same species of insects. It is impossible for two individuals to be completely identical in shape, but if the differences between individuals can be ensured to be relatively small, These differences will not affect the morphological identification, so it also proves that the individuals of this batch of Elephus sinensis are uniform. Typically, "in-bottle" homogeneity testing is often done in conjunction...
Embodiment 3
[0071] The four-striped bean elephant standard sample that above-mentioned embodiment makes is carried out definite value:
[0072] (1) Determination principles for the morphological characteristics of the standard sample of Elephant four-pattern
[0073] The standard sample of C. four-striped is confirmed by 5 experts in the field of insects to confirm whether the given standard sample of C. four-striped is C. four-striped.
[0074] According to the actual work and practical experience, the experts confirmed the morphological identification and description of the four-striped bean elephant.
[0075] (2) Morphological identification characteristics of the standard sample of C.
[0076] Body length 2.5-3.5mm, long oval, black or dark brown, with sparse golden yellow hairs. Tentacles zigzag, base five-segmented yellowish-brown, the rest black, or all yellowish-brown. The prothorax is trapezoidal, both sides are narrowed forward, and there is a pair of tumor protrusions in the...
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