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Callosobruchus maculates standard sample and preparation method thereof

A technology of the four-striped bean weed and standard samples, which is applied in the field of plant quarantine, can solve the problems of no insect standard sample research, identification results that cannot be traced, inedible, and used for planting, etc., to eliminate the difference in results and ensure accuracy and reliability. The effect of sex and good mobility

Inactive Publication Date: 2014-11-05
胡强 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The bean weevil larvae harm all kinds of beans in the field and in the warehouse, and turn the beans into empty shells, which cannot be eaten or used for planting, greatly reducing the value of the commodity
However, at present, standard samples are only widely used in metallurgy, non-ferrous metals, chemistry, food additives, etc., the research carried out is relatively in-depth, and the technology has matured, but it is still blank in the field of plant quarantine, and there is no research on insects and insects. In the study of standard samples, many laboratories still use traditional methods in the identification of insect morphology, comparing pictures or specimens preserved in the laboratory for identification, and these specimens come from different sources, and specimens preserved in different laboratories sometimes have morphological differences. Large variances, even wrong specimens
In this way, due to the differences in reference substances, there are great differences and incomparability in the identification results between different laboratories, and the identification results cannot be traced.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The preparation method of the four-striped bean elephant standard sample, the concrete steps are as follows:

[0042] The first step, the source of the standard sample

[0043] The source of the standard sample of Elephus sinensis was intercepted from the imported goods; the intercepted Elephus four-striped goods were identified by experts and raised as parents.

[0044]No matter which country or region they come from, the intercepted imported weeds should be kept in isolation. After one generation, observe whether the traits of the progeny are separated, and then select the parent as a standard sample. For the intercepted insects, it is necessary to pay attention to whether they are alive in order to prepare for the next step of propagation. Keep a record of intercepted insects, in particular clearly labeling the country from which the insect came from. According to the corresponding growth conditions of different insects, select the growth conditions such as tempera...

Embodiment 2

[0061] The four-lined bean elephant standard sample that above-mentioned embodiment makes carries out homogeneity and stability test:

[0062] (1) Uniformity test

[0063] The uniformity test is completed in two steps, one is the "in-bottle" uniformity test, and the other is the "between-bottle" uniformity test.

[0064] When designing the "in-bottle" uniformity inspection scheme, the main consideration is to minimize the differences among individual insects. Due to the diversity of organisms, it is inevitable that there will be some differences between each individual of the same species of insects. It is impossible for two individuals to be completely identical in shape, but if the differences between individuals can be ensured to be relatively small, These differences will not affect the morphological identification, so it also proves that the individuals of this batch of Elephus sinensis are uniform. Typically, "in-bottle" homogeneity testing is often done in conjunction...

Embodiment 3

[0071] The four-striped bean elephant standard sample that above-mentioned embodiment makes is carried out definite value:

[0072] (1) Determination principles for the morphological characteristics of the standard sample of Elephant four-pattern

[0073] The standard sample of C. four-striped is confirmed by 5 experts in the field of insects to confirm whether the given standard sample of C. four-striped is C. four-striped.

[0074] According to the actual work and practical experience, the experts confirmed the morphological identification and description of the four-striped bean elephant.

[0075] (2) Morphological identification characteristics of the standard sample of C.

[0076] Body length 2.5-3.5mm, long oval, black or dark brown, with sparse golden yellow hairs. Tentacles zigzag, base five-segmented yellowish-brown, the rest black, or all yellowish-brown. The prothorax is trapezoidal, both sides are narrowed forward, and there is a pair of tumor protrusions in the...

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PUM

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Abstract

The invention relates to the plant quarantine field. As for the Callosobruchus maculates standard sample, the determined Callosobruchus maculates polypide is embedded in the resin, each resin has a Callosobruchus maculates. In the Callosobruchus maculates standard sample, the Callosobruchus maculates is embedded in the resin, the resin raw material is liquid, has good flowability, and can sufficiently flood on the surface of the sample, the sample cannot be dissolved into water after being dried and cured as a film. The resin has extremely high transparency so as to conveniently observe fine structures in the standard sample. Furthermore, the resin has the good inflaming retarding, anti-aging, non-toxic, anti-oxidizing, heat-resisting and insulating performances; the Callosobruchus maculates specimen is embedded in the resin so as to delay the valid period of the standard sample and reduce the standard sample cost. The result difference caused by different references used in different laboratories can be eliminated; and meanwhile, the traceability of the authentication result can be guaranteed so as to guarantee that the accuracy and reliability of the Callosobruchus maculates appearance authentication result play a significant part.

Description

technical field [0001] The invention relates to the field of plant quarantine, in particular to a standard sample of Elephus chinensis, and also relates to a preparation method thereof. Background technique [0002] Four-striped bean elephant ( Bruchus maculatus (Fabricius)) is an important pest in the list of quarantine pests in my country. It was first discovered in the United States and has now spread to most countries and regions in the world. Four-striped bean elephants can reproduce and cause damage in fields and warehouses. It mainly causes serious harm to stored edible beans. Adults lay eggs on bean pods or bean grains, most of the larvae enter directly near the hilum, molt 7-12 days after entering the bean grains, thoracic feet degenerate, eat a lot of food, and cause serious damage, the boreholes on each kidney bean can reach 12 or more. The bean weevil larvae harm all kinds of bean grains in the field and in the warehouse, and the bean grains are bored into e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28
Inventor 胡强王有福殷国建侯国强
Owner 胡强