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Polypeptide Pm4 resisting cariogenic bacteria in oral cavity and preparation method thereof

A technology of antibacterial peptides and cariogenic bacteria, applied in the field of medicine and biology, can solve the problems of high synthesis cost and excessive length of natural antibacterial peptides

Inactive Publication Date: 2012-04-18
倪龙兴
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of anti-oral cariogenic bacteria polypeptide Pm4 and its preparation method, in order to overcome the problem that the length of natural antibacterial peptide is too long and the synthesis cost is relatively high

Method used

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  • Polypeptide Pm4 resisting cariogenic bacteria in oral cavity and preparation method thereof
  • Polypeptide Pm4 resisting cariogenic bacteria in oral cavity and preparation method thereof
  • Polypeptide Pm4 resisting cariogenic bacteria in oral cavity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0024] figure 1 It is the α-helical structure software prediction map of polypeptides Pm11 and Pm14.

[0025] figure 2 is an electron micrograph of peptide-treated bacteria. A, normal S. mutans; B, Pm11-treated S. mutans; C, Pm14-treated S. mutans; D, normal blood S. mutans; E, Pm11-treated S. mutans; F, Pm14-treated S. mutans Streptococcus mutans. It can be seen that the surface of the bacteria treated with the polypeptide changes, and the phenomenon of porous or bacterial disintegration and shrinkage appears.

[0026] 1. Design and synthesis of peptides

[0027] 1.1 Design of peptides

[0028] The polypeptide is designed by intercepting the active center of pleurocidin and deleting and replacing amino acids. The physical properties of the designed peptides are shown in Table 1. The parameters of the α-helical structure are shown in Table 1, and the schematic diagram is shown in figure 2 .

[0029]

[0030]

[0031] 1.2 Fmoc method to synthesize peptides.

[0...

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Abstract

The invention belongs to the field of medicinal biotechnology, and relates to a polypeptide Pm4 resisting cariogenic bacteria in an oral cavity and a preparation method thereof. The polypeptide Pm4 resisting cariogenic bacteria in an oral cavity is synthesized by a Fmoc chemical method. The preparation method comprises the following steps of searching an active center of a natural polypeptide pleurocidin, wherein the active center has a certain effect of resisting and killing common cariogenic bacteria in an oral cavity, and on that basis of determination of the active center, carrying out amino acid deletion and replacement to obtain the polypeptide Pm4 resisting cariogenic bacteria in an oral cavity, wherein the polypeptide Pm4 resisting cariogenic bacteria in an oral cavity is a novel polypeptide and has a sequence of GWGKFFKKFFKVGK; and the common cariogenic bacteria in an oral cavity comprise streptococcus mutans, streptococcus sobrinus and streptococcus sanguis. The polypeptide Pm4 resisting cariogenic bacteria in an oral cavity solves the problems that the natural polypeptide resisting bacteria is overlong and has a relatively high synthesis cost.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and relates to Fmoc chemically synthesized polypeptide, that is, a polypeptide Pm4 for resisting oral cariogenic bacteria and a preparation method thereof. The polypeptide has the ability of resisting oral cariogenic bacteria, especially bacteria of the genus Streptococcus. Background technique [0002] Although a lot of manpower and material resources have been invested, dental caries is still one of the three major non-communicable diseases in the world. Caries is a chronic and progressive bacterial infectious disease that occurs in dental hard tissues under the influence of various factors in the internal and external environment of the oral cavity. Streptococcus mutans and Streptococcus farus are the main cariogenic bacteria. In 1946, McClure and Hewitt confirmed that penicillin can inhibit the occurrence of dental caries in rats. The main mechanism is to inhibit the synthesis o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61P1/02A61P31/04
Inventor 倪龙兴陶睿
Owner 倪龙兴