Kit for noninvasive detection of ovarian cancer susceptible gene

A detection kit and susceptibility gene technology, applied in the field of molecular biology, can solve problems such as loss of body metabolism and reduction
CN102424853AInactive Publication Date: 2012-04-25解码(上海)生物医药科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
解码(上海)生物医药科技有限公司
Publication Date
2012-04-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a kit for noninvasive detection of an ovarian cancer susceptible gene. The kit comprises specific primers and DNA sequencing primers for detecting whether C677T and GSTT1 genes on MTHFR gene are null or present, and for detecting the genotype of 3 mononucleotide polymorphism sites of Leu432Val on CYP1B1 gene, a PCR (polymerase chain reaction) reaction assembly, a PCR product purification assembly, and a DNA sequencing reaction assembly, etc. The kit of the invention assesses the risk grade of ovarian cancer suffering by women through detecting the genotype of 3 mononucleotide polymorphism sites closely related to ovarian cancer occurrence, and finally guides women in targeted prevention of ovarian cancer occurrence in a gene level according to the gene detection result of each examinee, thus lowering the morbidity of ovarian cancer. The invention adopts an oral mucosa cell sampling method, which is painless and noninvasive, and avoids cross infection. The kit of the invention has precise and reliable detection results, so that there is no need to purchase expensive special imported equipment, and the method of the kit is very easy to popularize and promote.
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Description

Technical field

[0001] The invention belongs to the field of molecular biology, and relates to medicine and biotechnology, and specifically relates to a ovarian cancer susceptibility gene detection kit, which evaluates the risk level of ovarian cancer from the genetic level according to the detection results, and serves as a direction for the prevention and treatment of ovarian cancer guide. Background technique

[0002] Ovarian cancer is one of the common tumors of female reproductive organs, the incidence rate is second only to cervical cancer and uterine body cancer and ranks third. However, the deaths caused by ovarian cancer are the first of all types of gynecological tumors, posing a serious threat to women’s lives. Ovarian cancer is the gynecological malignant tumor with the highest mortality rate because of its insidious onset and rapid progress. 70%-80% of ovarian cancer patients are already at an advanced stage when discovered, and the 5-year survival rate is only 20%-...

Claims

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