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Preparation method of piper laetispicum extractive, extractive and application thereof

A technology of konjac and extract, which is applied in the field of preparation of konjac extract, can solve problems such as unsatisfactory antidepressant activity, and achieve the effects of increasing alkaloid content and optimizing process conditions

Inactive Publication Date: 2012-05-02
王全福
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From the experimental results disclosed in this patent application, it can be known that the antidepressant activity of the extract is not ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Preparation of extract of konnyaku

[0019] According to Table 1, take the roots, rhizomes, canes, leaves and fruits or the whole plant of konjac grandiflora, respectively, to prepare the extracts of konjac grandiflora, as follows:

[0020] Sample 1: Take the roots and canes of the konjac leaf and make coarse powder, add about 10 times the amount of 70% ethanol of the medicinal material, and percolate at room temperature to obtain the crude extract solution, and then concentrate the crude extract solution under vacuum to About 25% of the original volume, the concentrated crude extract solution is stirred evenly and then applied to a non-polar macroporous adsorption resin column for separation and purification, followed by elution with 30% and 80% ethanol, and collect the 80% ethanol eluted liquid After concentration and drying, a refined extract is obtained.

[0021] Sample 2: Take the cane and leaf cuts of konjac, add about 5 times the amount of the medicinal mater...

Embodiment 2

[0031] Example 2 Antidepressant activity-forced swimming test in mice

[0032] Mice (KM mice, male, 22-25g) were randomly divided into groups, 10 in each group, and each group was administered A (sample 1 of Example 1) and B (sample 2 of Example 1) at the indicated doses. ), C (sample 6 of Example 1), D (sample 7 of Example 1), once a day for 14 consecutive days, with physiological saline as a control, and a positive control for fluoxetine. One hour after the administration on the 14th day, the mice were placed in a glass cylinder with a diameter of 14 cm×h20 cm and a water depth of 10 cm (water temperature 25° C.) to swim. Starting from the second minute, the accumulated immobility time within 4 minutes was recorded, and the results are shown in Table 2.

[0033] Table 2. Results of forced swimming test in mice

[0034] Drugs

Dose (mg / kg / d)

Accumulated immobile time (s)

CK-

-

161.3±16.4

CK+

20

54.5±12.6**

A

20

49.4±13.3**

A

10

63.5±11.2**

A

5

115....

Embodiment 3

[0039] Example 3 Antidepressant activity-mouse tail suspension test

[0040] Mice (KM mice, male, 22-25g) were randomly divided into groups, 10 in each group, and each group was administered A (sample 1 of Example 1) and B (sample 2 of Example 1) at the indicated doses. ), C (Sample 6 of Example 1), D (Sample 7 of Example 1), once a day for 14 consecutive days, with physiological saline as a control, and a positive control for venfalacine. The mouse tail suspension test was performed 1 hr after the last administration, and the duration was 6 min. The time of standing still within 6 min was recorded. The results are shown in Table 3.

[0041] Table 3. Results of mouse tail suspension test

[0042] Drugs

Dose (mg / kg / d)

Accumulated immobile time (s)

CK-

-

124.8±56.7

CK+

50

43.7±31.8**

A

10

49.1±25.3**

A

5

67.4±29.6*

B

10

51.5±23.1**

B

5

62.7±39.3*

C

10

69.5±31.5*

C

5

84.4±35.1*

D

10

76.3±28.4*

D

5

95.2±42.6

[0043] CK-: ...

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PUM

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Abstract

The invention discloses an improved and optimized preparation method of piper laetispicum extractive, extractive obtained by the method and application of the extractive used as a neurotransmitters reuptake inhibitor in treating and / or preventing nervous and mental diseases associated with monamine transmitter in the fields of medical treatment, healthcare and food additive and the like. Compared with the piper laetispicum extractive obtained by the existing preparation method, the piper laetispicum extractive obtained by the preparation method of the invention remarkably increases the antidepressant activity and synchronously reduces the acute toxicity to a mouse further; and moreover, the extractive obtained by the method provided by the invention remarkably increases the security when being used as medicine, and further strengthens the effectiveness.

Description

Technical field [0001] The present invention relates to Piper laetispicum C.DC., a medicinal plant of the genus Piper, in particular to a method for preparing an extract of Piper laetispicum, an extract prepared by the method, and the application of the extract. Background technique [0002] Diseases related to the metabolism of monoamine transmitters such as serotonin (5-HT), norepinephrine (NA) and dopamine (DA) have become one of the most important diseases that hinder human health. According to the data of WHO, The total disease burden caused by it is second only to coronary heart disease in developed countries. Neuropsychiatric diseases rank first in the total disease burden in my country, accounting for about 1 / 5 of the total disease burden. For example, numerous community and medical studies have revealed that depression and anxiety disorders have a relatively high incidence. The National Syndrome Survey found that 17.3% of the general population suffers from a depressive e...

Claims

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Application Information

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IPC IPC(8): A61K36/67A61P25/18A61P25/00A61P25/24A61P25/22A61P25/20A61P25/08A61P25/16A61P25/28A23L1/30
Inventor 王全福
Owner 王全福
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