Preparation method of piper laetispicum extractive, extractive and application thereof
A technology of konjac and extract, which is applied in the field of preparation of konjac extract, can solve problems such as unsatisfactory antidepressant activity, and achieve the effects of increasing alkaloid content and optimizing process conditions
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Embodiment 1
[0018] Example 1 Preparation of extract of konnyaku
[0019] According to Table 1, take the roots, rhizomes, canes, leaves and fruits or the whole plant of konjac grandiflora, respectively, to prepare the extracts of konjac grandiflora, as follows:
[0020] Sample 1: Take the roots and canes of the konjac leaf and make coarse powder, add about 10 times the amount of 70% ethanol of the medicinal material, and percolate at room temperature to obtain the crude extract solution, and then concentrate the crude extract solution under vacuum to About 25% of the original volume, the concentrated crude extract solution is stirred evenly and then applied to a non-polar macroporous adsorption resin column for separation and purification, followed by elution with 30% and 80% ethanol, and collect the 80% ethanol eluted liquid After concentration and drying, a refined extract is obtained.
[0021] Sample 2: Take the cane and leaf cuts of konjac, add about 5 times the amount of the medicinal mater...
Embodiment 2
[0031] Example 2 Antidepressant activity-forced swimming test in mice
[0032] Mice (KM mice, male, 22-25g) were randomly divided into groups, 10 in each group, and each group was administered A (sample 1 of Example 1) and B (sample 2 of Example 1) at the indicated doses. ), C (sample 6 of Example 1), D (sample 7 of Example 1), once a day for 14 consecutive days, with physiological saline as a control, and a positive control for fluoxetine. One hour after the administration on the 14th day, the mice were placed in a glass cylinder with a diameter of 14 cm×h20 cm and a water depth of 10 cm (water temperature 25° C.) to swim. Starting from the second minute, the accumulated immobility time within 4 minutes was recorded, and the results are shown in Table 2.
[0033] Table 2. Results of forced swimming test in mice
[0034] Drugs
Dose (mg / kg / d)
Accumulated immobile time (s)
CK-
-
161.3±16.4
CK+
20
54.5±12.6**
A
20
49.4±13.3**
A
10
63.5±11.2**
A
5
115....
Embodiment 3
[0039] Example 3 Antidepressant activity-mouse tail suspension test
[0040] Mice (KM mice, male, 22-25g) were randomly divided into groups, 10 in each group, and each group was administered A (sample 1 of Example 1) and B (sample 2 of Example 1) at the indicated doses. ), C (Sample 6 of Example 1), D (Sample 7 of Example 1), once a day for 14 consecutive days, with physiological saline as a control, and a positive control for venfalacine. The mouse tail suspension test was performed 1 hr after the last administration, and the duration was 6 min. The time of standing still within 6 min was recorded. The results are shown in Table 3.
[0041] Table 3. Results of mouse tail suspension test
[0042] Drugs
Dose (mg / kg / d)
Accumulated immobile time (s)
CK-
-
124.8±56.7
CK+
50
43.7±31.8**
A
10
49.1±25.3**
A
5
67.4±29.6*
B
10
51.5±23.1**
B
5
62.7±39.3*
C
10
69.5±31.5*
C
5
84.4±35.1*
D
10
76.3±28.4*
D
5
95.2±42.6
[0043] CK-: ...
PUM
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