Method for screening anti-angiogenesis active substances
An anti-angiogenesis and angiogenesis technology, applied in biological testing, material testing, etc., can solve the problems of affecting angiogenesis, restricting the screening of anti-angiogenic active substances, single target, etc., to improve objectivity and accuracy, The effect of reducing experimental cost and low cost
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Embodiment 1
[0035] Example 1: Effect of PTK787 on Zebrafish Angiogenesis
[0036] Select the normal embryos that have developed for 24 hours under a stereomicroscope, and transfer them to the sample wells of a 96-well plate, one embryo per well. PTK787 solutions containing different concentrations have been added to the sample wells in advance, with 10 replicates for each concentration. The control group was water for embryo culture. Then cover and seal, place in light incubator (28°C), let the embryo continue to develop. After 48 hours of embryonic development, observe the shape of the heart under an inverted microscope, record the heartbeat frequency, and then observe and count the number of vessels with blood flow between body segments to obtain the number of intersegmental blood flow in the experimental group and the control group. The results are shown in Table 1, and the angiogenesis inhibition rate was calculated according to the following formula:
[0037]
[0038] At the co...
Embodiment 2
[0043] Example 2: Effects of Indirubin on Zebrafish Angiogenesis
[0044]Select the normal embryos that have developed for 24 hours under a stereomicroscope, and transfer them to the sample wells of a 96-well plate, one embryo per well. Indirubin solutions containing different concentrations have been added to the sample wells in advance, and each concentration has 10 repetitions. The control group was water for embryo culture. Then cover and seal, place in light incubator (28°C), let the embryo continue to develop. After 48 hours of embryonic development, observe the shape of the heart under an inverted microscope, record the heartbeat frequency, and then observe and count the number of vessels with blood flow between body segments to obtain the number of intersegmental blood flow in the experimental group and the control group. The results are shown in Table 2, and the angiogenesis inhibition rate was calculated according to the following formula:
[0045]
[0046] Ind...
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