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Method for screening anti-angiogenesis active substances

An anti-angiogenesis and angiogenesis technology, applied in biological testing, material testing, etc., can solve the problems of affecting angiogenesis, restricting the screening of anti-angiogenic active substances, single target, etc., to improve objectivity and accuracy, The effect of reducing experimental cost and low cost

Active Publication Date: 2012-05-09
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method is simple and fast, but the target is single. Only samples that affect the expression of VEGFR2 (type II vascular endothelial growth factor receptor) can produce positive results, and samples that affect the expression of VEGFR2 may not necessarily affect angiogenesis
The above examples confirm the effectiveness of using zebrafish to screen anti-angiogenic drugs, but the shortcomings of the above models greatly limit the screening of anti-angiogenic active substances

Method used

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  • Method for screening anti-angiogenesis active substances
  • Method for screening anti-angiogenesis active substances
  • Method for screening anti-angiogenesis active substances

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Effect of PTK787 on Zebrafish Angiogenesis

[0036] Select the normal embryos that have developed for 24 hours under a stereomicroscope, and transfer them to the sample wells of a 96-well plate, one embryo per well. PTK787 solutions containing different concentrations have been added to the sample wells in advance, with 10 replicates for each concentration. The control group was water for embryo culture. Then cover and seal, place in light incubator (28°C), let the embryo continue to develop. After 48 hours of embryonic development, observe the shape of the heart under an inverted microscope, record the heartbeat frequency, and then observe and count the number of vessels with blood flow between body segments to obtain the number of intersegmental blood flow in the experimental group and the control group. The results are shown in Table 1, and the angiogenesis inhibition rate was calculated according to the following formula:

[0037]

[0038] At the co...

Embodiment 2

[0043] Example 2: Effects of Indirubin on Zebrafish Angiogenesis

[0044]Select the normal embryos that have developed for 24 hours under a stereomicroscope, and transfer them to the sample wells of a 96-well plate, one embryo per well. Indirubin solutions containing different concentrations have been added to the sample wells in advance, and each concentration has 10 repetitions. The control group was water for embryo culture. Then cover and seal, place in light incubator (28°C), let the embryo continue to develop. After 48 hours of embryonic development, observe the shape of the heart under an inverted microscope, record the heartbeat frequency, and then observe and count the number of vessels with blood flow between body segments to obtain the number of intersegmental blood flow in the experimental group and the control group. The results are shown in Table 2, and the angiogenesis inhibition rate was calculated according to the following formula:

[0045]

[0046] Ind...

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Abstract

The invention relates to a method for screening anti-angiogenesis active substances, in particular to a method for screening the anti-angiogenesis active substances by utilizing a zebrafish model, which belongs to the field of drug screening. According to the invention, whether the substances to be screened have an inhibiting effect on angiogenesis is obtained through observation and statistics of quantity of blood vessels with blood flow in internodium of a zebrafish body. Compared with the prior art that the influence of a compound / mixture on angiogenesis is reflected by the existence or the completeness of a blood vessel wall, the method for reflecting the existence of the blood vessels in a blood vessel function perspective is more scientific and objective, and the accuracy of the result is improved.

Description

technical field [0001] The invention relates to a method for screening anti-angiogenesis drugs, in particular to a method for screening anti-angiogenesis drugs by using a zebrafish model, and belongs to the field of drug screening. Background technique [0002] Angiogenesis is a necessary process in the occurrence and development of malignant tumors, and is closely related to the growth and metastasis of malignant tumors. The role of tumor blood vessels on malignant tumors is mainly manifested in two aspects. 1. Tumor blood vessels provide the necessary nutrients and oxygen for the growth of tumor cells; premise. In 1971, Folkman first proposed that tumor growth and metastasis are dependent on blood vessels, and blocking angiogenesis is an effective strategy to curb tumor growth. This famous theory made people begin to realize the importance of blood vessels to tumors. [0003] Anti-angiogenesis has become one of the effective strategies to treat tumors. Establishing a re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
Inventor 刘可春何秋霞袁延强韩利文彭维兵陈锡强王雪王希敏侯海荣
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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