Identification and application of specific expression promoter of plant root

Abstract

The invention discloses a method for regulating the expression of an exogenous nucleotide sequence in a plant body and a DNA (Deoxyribonucleic Acid) construct. The DNA construct contains a new nucleotide sequence of a specific expression promoter of a plant root. The invention further provides a method for preferentially expressing the exogenous nucleotide sequence in the plant root by using the specific expression promoter of the root, disclosed by the invention.

Description

technical field [0001] The invention belongs to the technical fields of plant bioengineering and plant improvement genetic engineering, and specifically relates to the isolation, identification and application of a plant root-specific expression promoter. Background technique [0002] The exogenous DNA sequence is linked to a specific promoter to promote expression in the plant host, and the choice of the promoter type determines the time and location of gene expression. At present, some strong constitutive promoters are widely used in the field of agricultural biotechnology, such as the CaMV 35S promoter and the maize Ubiquitin-1 promoter (Battraw and Hall, 1990; Christensen et al.1992). When sub-inducing the target gene to transform crops such as rice in order to improve the quality of the crop, the improvement effect is often not obvious because the time (developmental stage specificity) or space (tissue and organ specificity) of the target gene expression cannot be well ...

AI Technical Summary

Problems solved by technology

At present, some strong constitutive promoters are widely used in the field of agricultural biotechnology, such as the CaMV 35S promoter and the maize Ubiquitin-1 promoter (Battraw and Hall, 1990; Christensen et al.1992). When sub-inducing the target gene to transform crops such as rice in order to improve the quality of the crop, the improvement effect is often not obvious because the time (developmental stage specificity) or space (tissue and organ specificity) of the target gene expression cannot be well controlled, or Because these constitutive promoters induce too high gene expression and affect the growth and development of plants, these are the obstacles encountered when using constitutive strong promoters combined with functional genes to improve crop quality.

[0003] In addition, when studying certain metabolic processes or regulatory pathways, it is often necessary to transform two or more genes on the same pathway into the same line, transforming one of the genes to obtain a transgenic plant and then transforming another gene, or It takes a long time to wait for the hybridization after the two genes have been transformed separately. In order to improve the efficiency and shorten the time for transforming multiple genes, it has recently been reported that a new vector can be used to simultaneously transform multiple genes (Lin et al. 2003; Chen et al.2006), but if the same promoter is used repeatedly during multigene transformation, gene silencing may also occur due to the high homology of the promoter sequence

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