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Application of anti-sense miRNA (Ribonucleic Acid)-210 to preparation of anti-cancer drug

A miRNA-210, anticancer drug technology, applied in the field of biomedical materials to achieve the effect of promoting apoptosis of human glioma cells

Inactive Publication Date: 2012-05-30
DALIAN CHUANGDA TECH TRADE MARKET
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Problems solved by technology

But so far there is no literature report about the use of antisense miRNA-210 as a drug for the treatment of human glioma cell line invasion

Method used

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  • Application of anti-sense miRNA (Ribonucleic Acid)-210 to preparation of anti-cancer drug
  • Application of anti-sense miRNA (Ribonucleic Acid)-210 to preparation of anti-cancer drug
  • Application of anti-sense miRNA (Ribonucleic Acid)-210 to preparation of anti-cancer drug

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[0019] value. Treat with RNase-free DNase I and purify total RNA with QIAGEN RNeasy kit. See the kit manual for detailed operating principles and methods. Evaluate the quality of total RNA by agarose gel electrophoresis, observe and take pictures on a gel imager, and save the image. It is generally believed that 28S:18S>2 can preliminarily determine that the quality of total RNA is better.

[0020] 2. Preparation of MicroRNA chip: Firstly, obtain the sequence of prepared miRNA probe from the miRNA information logging website http: / / microrna.sanger.ac.uk (2005.09), including 313 human and 34 mouse miRNA sequences probe sequence. At the same time, we also published the article on XIE in Nature in 2005 (Xie X, Lu J, Kulbokas EJ, et al. Systematic discovery of regulatory motifs in humanpromoters and 3 UTRs by comparison of several mammals[J]. Nature, 2005, 434(7031):338), the probes corresponding to the 122 predicted miRNAs were also integrated into it, and finally 469 probes ...

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Abstract

The invention relates to an application of anti-sense miRNA (Ribonucleic Acid)-210to the preparation of an anti-cancer drug, in particular to an application of anti-sense miRNA-210. The anti-sense miRNA-210 for preparing the anti-cancer drug belongs to the technical field of biomedical materials. In the application, a miRNA chip is adopted to research the expression situation of three examples of brain glioma miRAN, nine examples of brain glioma samples and four strains of brain glioma cells are verified by a chip result with Northern and q-PCR (Polymerase Chain Reaction) methods and the like, and a result proves that the anti-sense miRNA-210 can obviously lower the invasion capability of the brain glioma cell strains.

Description

technical field [0001] The invention relates to the use of small RNA molecules, in particular to the use of antisense miRNA-210 for preparing anticancer drugs, and belongs to the technical field of biomedical materials. Background technique [0002] In recent years, a non-coding single-stranded RNA small molecule (microRNA, miRNA) widely exists in cells from plants, nematodes to humans, and they can be incompletely complementary to specific regions of the 3' untranslated end of their target mRNAs. interact and inhibit protein synthesis. In 1993, Lee et al. cloned the lin-4 gene for the first time in C. elegans using the classical cloning method, and confirmed the existence of small molecule RNA by the method of point mutation. In 2000, Reinhart et al. discovered the existence of the let-7 gene in humans and Drosophila, and speculated that this small molecule may be a regulatory molecule that is evolutionarily conserved and plays an important role in the life process. Subse...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61P35/00A61P25/00
Inventor 王爽
Owner DALIAN CHUANGDA TECH TRADE MARKET
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