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Method for preparing polyinosinic preparation and application of polyinosinic preparation to tumor resistance

A technology of polyinosinic acid and anti-tumor, which is applied in the field of polyinosinic acid preparation and its anti-tumor, and can solve the problems of not knowing polyinosinic acid, etc.

Inactive Publication Date: 2012-06-13
TIANJIN JIMINGSHENG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, it is still completely unknown whether polyinosinic acid, polycytidylic acid itself, and their complexes with cationic liposomes can induce cancer cell death, thereby killing cancer cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Add 40 g of glucose dissolved in 100 mL of water for injection to 2 g of refined egg yolk lecithin, stir and mix, and disperse with a homogenizer for 5 minutes to obtain a coarse dispersion of the carrier. Then use a small-scale emulsification disperser for experiments to disperse the above-mentioned crude dispersion for 1 hour, and after the volume is adjusted to 250 mL with water for injection, the carrier dispersion is recovered. While stirring, 150 mL of an aqueous solution containing 120 mg of polyinosinic acid, 120 mg of polycytidylic acid and 320 mg of kanamycin was added to 250 mL of the carrier dispersion, and the dispersion treatment was carried out for 1 hour with a small emulsification disperser for experiments again to obtain poly Myocyte preparations. Finally, the polyinosin preparations were respectively injected into 1 mL vials, and freeze-dried preparations were prepared according to conventional methods.

Embodiment 2

[0022] Add 4 kg of glucose dissolved in 10 L of water for injection to 30 g of egg yolk phospholipid, and after dispersing for 10 minutes with a high-pressure homogenizer, set the volume to 20 L with water for injection, and recover the carrier dispersion. While stirring, add 12L of an aqueous solution containing 10g of polyinosinic acid, 10g of polycytidylic acid, and 26g of kanamycin to 20L of the carrier dispersion, adjust the pH to 7.2 with hydrochloric acid, and then use a high-pressure homogenizer again to After dispersing for 30 minutes, the polykinocyte preparation was obtained. Then, the polyinosin preparations were respectively injected into 20 mL vials, and freeze-dried preparations were prepared according to conventional methods. The freeze-dried preparation was soluble in commercially available 5% dextrose infusion (500 mL).

Embodiment 3

[0024] Add 20 g of glucose dissolved in 100 mL of water for injection to 2 g of soybean lecithin, stir and mix, and disperse with a homogenizer for 5 minutes to obtain a coarse dispersion of the carrier. After the above-mentioned crude dispersion was dispersed for 1 hour with a small-scale high-pressure emulsifying disperser for experiments, the volume was adjusted to 250 mL with water for injection, and the carrier dispersion was recovered. While stirring, add 150 mL of an aqueous solution containing 60 mg of polyinosinic acid, 60 mg of polycytidylic acid, and 160 mg of kanamycin to 250 mL of the carrier dispersion, and then use a small-scale high-pressure emulsification disperser for the experiment to carry out dispersing treatment for 1 hour to obtain Polymyocyte preparations.

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Abstract

The invention aims to provide a method for preparing a polyinosinic preparation and application of the polyinosinic preparation to tumor resistance. The invention further provides a double-stranded ribonucleic acid (RNA) medicine containing polyinosinic acid and polycytidylic acid. The polyinosinic preparation contains a carrier in which phospholipids serve as an essential component, and a complex of the polyinosinic acid, the polycytidylic acid and kanamycin.

Description

Technical field: [0001] The present invention relates to a preparation method and anti-tumor application of a poly-inogenic preparation, in particular to a poly-inogenic preparation containing kanamycin and lecithin and an anti-tumor application. Background technique: [0002] Polyinosinic acid is a double-stranded RNA of polyribonucleotide copolymer composed of polyinosinic acid and polycytidylic acid. It is well known that it is a drug with strong interferon-inducing activity and immune strengthening effect. Since poly myocytes have an immune strengthening effect, they can indirectly inhibit the proliferation of cancer cells through immune responses, and their possibility as a cancer therapeutic agent is currently being studied. However, there has not yet been developed a poly-inosin cancer therapeutic agent that can effectively inhibit the proliferation of cancer cells by utilizing the indirect effect of the poly-inosin immune response, and no other indications based on i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/80A61K47/26A61K47/24A61P35/00
Inventor 焦月侯本晶王国新王智佳沈丽丽
Owner TIANJIN JIMINGSHENG BIOLOGICAL TECH
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