Method for determining content difference of rution in asparagus plants

Abstract

The invention discloses a determination method of rutin content differences in asparagus plants and belongs to the field of determination of bioactive substance in plants. The method comprises the following steps of: carrying out sex discrimination on different varieties of asparagus plants to be detected, taking roots, tender stems and leaves of the identified asparagus plants to respectively prepare sample solutions, carrying out high-performance liquid chromatography analysis on all sample solutions and control solutions respectively to obtain spectral peaks of rutin contents in asparagus plants of all varieties, and comparing rutin content differences by calculating spectral peak area. The method provided by the invention can be adopted to carry out rutin content determination on different parts of asparagus male and female plants of different varieties and provides theoretical basis for the enhancement of extraction rate of rutin in asparagus.

Description

Technical field [0001] The invention relates to the field of determination of biologically active substances in plants, in particular to a method for determining the difference in rutin content in asparagus plants. Background technique [0002] Asparagus, the scientific name of Asparagus, is unique in flavor, rich in nutrition, and has the magical effect of preventing cancer and cancer. It is known as the "King of Vegetables". This is mainly because it contains a variety of biologically active substances that regulate human physiological functions and prevent chronic diseases. Among them, rutin is widely concerned because of its unique functions and effects. Rutin, also known as rutin, is an important part of vitamin P. It has the function of maintaining normal osmotic pressure of blood vessels and reducing blood vessel fragility. It is mainly used in clinical practice. For adjuvant treatment of arteriosclerosis and hypertension. At present, the main raw material for extracting...

AI Technical Summary

Problems solved by technology

At present, the main raw material for extracting rutin in China is Huai Mi, and some are extracted from tartary buckwheat. In recent years, attempts have been made to extract rutin from Asparagus (asparagus), but the distribution of rutin in different varieties of asparagus is uneven, and the distribution of male and female plants is uneven. It is even more varied, which makes the extraction rate of rutin in asparagus low, which brings great inconvenience to the extraction and refining of rutin, and cannot fully meet the market demand.

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