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Method for building rat model of chronic pyelonephritis by perfusing Escherichia coli into bladder

A technology of Escherichia coli and chronic pyelonephritis, which is applied in the field of establishing rat models of chronic pyelonephritis, and can solve problems such as immaturity of the model

Inactive Publication Date: 2012-06-20
王怡
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Problems solved by technology

Therefore, these models are not mature enough

Method used

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  • Method for building rat model of chronic pyelonephritis by perfusing Escherichia coli into bladder

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Embodiment 1

[0015] 1. Experimental animals

[0016] 20 13-month-old normal clean SD female rats, weighing 200g-215g, were provided by Shanghai Slack Experimental Animal Co., Ltd., and were reared at room temperature in the clean animal experiment center of Yueyang Hospital of Integrated Traditional Chinese and Western Medicine affiliated to Shanghai University of Traditional Chinese Medicine. 7 days.

[0017] 2. Experimental reagents

[0018] Escherichia coli O111B4 (ATCC 29522, China Institute for the Control of Pharmaceutical and Biological Products, batch number: 44155).

[0019] 3. Experimental grouping

[0020] 13-month-old SD female SD rats were randomly divided into two groups, ① control group, n=10; ② model group, n=10, and there was no statistical difference in body weight between the groups.

[0021] 4. Model method:

[0022] 4.1 Intravesical perfusion method of Escherichia coli:

[0023] (1) Preparation of Escherichia coli solution: Under aseptic conditions, take out the D...

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Abstract

A method for building a rat model of chronic pyelonephritis by perfusing Escherichia coli into bladder includes the following steps of disinfecting the urethral orifice of the anaesthetized rat by the benzalkonium bromide for six weeks and twice each week, inserting a steel wire into a 24G intravenous indwelling catheter with the angle of 15-30 degrees, lubricating the intravenous indwelling catheter with paraffin oil, inserting the intravenous indwelling catheter into the urethral orifice of the rate until reaching the 3cm position of the inside of the bladder, removing the steel wire, draining and discarding urine, injecting Escherichia coli suspension with the concentration ranging from 108-109FU / 100ul by a 1ml OT needle tube, removing the catheter, and finally placing the rat into a rat cage until the rat revives naturally. The rat model of chronic pyelonephritis built by the method has the advantages that infiltration with lymphocytes and plasmocytes can be observed in renal interstitium evidently, infiltration with a small quantity of polymorphonuclear leucocytes and congestion and edema of blood capillary can also be observed after kidney pathological sections of the rat are subjected to HE(Hematoxylin and Eosin) staining.

Description

technical field [0001] The invention relates to a method for establishing a rat chronic pyelonephritis model, which is used for simulating, researching and preventing human chronic pyelonephritis and other medical fields. Background technique [0002] Urinary tract infection is a common clinical disease and frequently-occurring disease. According to the statistics of our national census, its incidence rate accounts for 0.91% of the population. It has a high incidence rate among women, children, the elderly, HIV-infected patients, diabetes and tumor patients. . Despite the rapid development of anti-disease drug research and the continuous updating of varieties, the incidence of urinary tract infection is still high, and it is constantly facing new challenges. The abuse of anti-viruses and the application of immunosuppressants have caused some original non-pathogenic viruses to Infections are on the rise. Urinary tract infection is divided into urethritis, cystitis and pyelo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D7/00A01K67/027
Inventor 王怡顾向晨陈敏
Owner 王怡
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