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Method for extracting coixol from rhizoma phragmitis

A kind of technology of coixin and reed root, applied in biological field

Inactive Publication Date: 2013-10-30
辽宁仙鹤矿泉水有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, no one has extracted coixin from reed root, and coixin has many functions, so the research on coixin from reed root is very meaningful

Method used

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  • Method for extracting coixol from rhizoma phragmitis
  • Method for extracting coixol from rhizoma phragmitis
  • Method for extracting coixol from rhizoma phragmitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 The method of extracting coixin from reed rhizome

[0021] (1) Preparation method

[0022] 1) Pre-treatment of reed rhizome: rinse fresh reed rhizome with tap water, rinse once with distilled water, drench the water, and dry in a constant temperature blast drying oven at 60°C for 6 hours. Cut into small sections then, pulverize with pulverizer, the reed rhizome powder of pulverizing is filtered through 30 mesh sieves, get the reed rhizome powder under the sieve for subsequent use.

[0023] 2) Selection of extraction solvent: Select six extraction solvents according to Table 1, and perform Soxhlet extraction respectively.

[0024] 3) Steps of extracting coixin:

[0025] a) Weigh reed rhizome powder, put it into a Soxhlet extractor, according to the ratio of solid to liquid 1:15 (that is, add 15ml of extraction solvent to 1g reed root powder), add extraction solvent as shown in Table 1, and extract for 2h; b) Extraction solution Concentrate by rotary evapor...

Embodiment 2

[0034] Example 2 The method of extracting coixin from reed rhizome

[0035] (1) Preparation method:

[0036] 1) Pretreatment of reed rhizome: same as in Example 1.

[0037] 2) Selection of extraction solvent: choose and add acetone with different solid-liquid ratios according to Table 2, and perform Soxhlet extraction respectively.

[0038] 3) Steps of extracting coixin:

[0039] a) Weigh a certain amount of reed rhizome powder, put it into a Soxhlet extractor, add acetone solvent according to the solid-liquid ratio in Table 2, and extract for 2 hours; b) Concentrate by rotary evaporation (40°C, 0.09Mpa), evaporate in a water bath at 60°C Dry the solvent to obtain a concentrated solution; c) Dissolve the concentrated solution in methanol and store it in a refrigerator at 4°C.

[0040] (2) Detection

[0041] 1) Separation by thin layer chromatography, the developing solvent is petroleum ether / ethyl acetate=5.5:1. see results figure 1 . from figure 1 It can be seen tha...

Embodiment ３

[0048] Example 3 The method of extracting coixin from reed root

[0049] (1) Preparation method:

[0050] 1) Pretreatment of reed rhizome: same as in Example 1.

[0051] 2) Selection of extraction solvent: Acetone is used as the extraction solvent.

[0052] 3) Steps of extracting coixin:

[0053] a) Weigh a certain amount of reed rhizome powder, put it into a Soxhlet extractor, add acetone solvent at a solid-liquid ratio of 1:20, and extract according to the time shown in Table 3; b) Concentrate by rotary evaporation (40°C , 0.09Mpa), the solvent was evaporated in a water bath at 60°C to obtain a concentrated solution; c) the concentrated solution was dissolved in methanol and stored in a refrigerator at 4°C.

[0054] (2) Detection

[0055] 1) Separation by thin layer chromatography, the developing solvent is petroleum ether / ethyl acetate=5.5:1. Through thin-layer chromatography, it can be seen that the substance with the same Rf value (Rf=0.44) as that of the standard ...

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Abstract

This invention relates to a method for extracting coixol from rhizoma phragmitis, comprising the following steps according to the technical scheme: (a) weighting a certain amount of rhizoma phragmitis powder, putting into a Soxhlet extractor, adding an extraction solvent according to the proportion of 1:15-35, and extracting for 2-6h; and (b) carrying out rotary evaporation and concentration at the temperature of 40 DEG C and the pressure of 0.09Mpa, and drying the solvent by volatilizing through water bath at 60 DEG C. The thin-layer chromatography and the high performance liquid chromatography (HPLC) adopted for qualitative and quantitative analysis prove that the coixol extracted from the rhizoma phragmitis is really obtained. The standard curve line Y is obtained by adopting the HPLC and presents a good linear relationship in the range from 0.1mug to 0.5mug, wherein Y=217009X+12086 (R2=0.9994). The method for extracting the coixol from the rhizoma phragmitis achieves the average recovery rate of 96.35% and the relative standard deviation (RSD) of 0.16%. Due to the adoption of the method for extracting the coixol from the rhizoma phragmitis, the rhizoma phragmitisa coixol is studied for the first time, and the development and the utilization of the coixol are made possible.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting coixin from reed root. Background technique [0002] Coixin is also known as coixamide. It is a carbocyclic aromatic acid phenolic compound. Needle crystal (water), melting point 151.5~152.5℃. Colorless needle crystal (acetone-petroleum ether), melting point 157~158℃. Coixin has an inhibitory effect on skeletal muscle and central nervous system. Animal experiments have shown that it has sedative, analgesic, anti-inflammatory, cooling and antipyretic effects, inhibition of polysynaptic reactions, muscle relaxation, anti-convulsion, and increases the amplitude and frequency of brain waves. , Inhibit the muscle contraction of frogs, the anaerobic glycolysis of glycogen and other pharmacological effects. According to clinical trials of modern Chinese and Western medicine, at an appropriate concentration, it can also be used to improve mood, enhance...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D263/58
Inventor 刘宏生赵健邵丽杰朱俊丰
Owner 辽宁仙鹤矿泉水有限公司
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