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Method for identification of fossils through utilizing chemical component fingerprint technology

A fingerprint and chemical composition technology, applied in the field of auxiliary identification of fossils, can solve problems such as unreported

Active Publication Date: 2012-06-27
BEIJING HUAMIAO TRADITIONAL CHINESE MEDICINE ENG TECH DEV CENT
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  • Summary
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Problems solved by technology

These technologies have been widely used in the identification of medicines, health products, agricultural products and food (Chai Yifeng et al. Pharmaceutical Services and Research, 2006, 6 (4): 241-245; Zhang Lianyi et al. Animal Husbandry and Feed Science, 2007, (1 ): 43), but the application of this technique in fossil identification has not been reported

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  • Method for identification of fossils through utilizing chemical component fingerprint technology

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Embodiment

[0011] Take 5 mg of eucommia seed fossils and modern eucommia seeds respectively, put them in 5 ml glass test tubes, add 1 ml of chromatographically pure methanol, and ultrasonically extract for 30 minutes. A phase chromatograph with an injection volume of 10 μl. The chromatographic conditions are as follows: Agilent C 18 ODS chromatographic column (250mm×4.6mm, 5μm), the mobile phase is acetonitrile-0.5% acetic acid solution (0→50min: 5∶98→50∶50), the flow rate is 1.0mL min -1 , the detection wavelength is 210nm. Through the above experimental process, the high performance liquid chromatograms of Eucommia ulmoides seed fossils and modern eucommia seeds were respectively obtained. Through the analysis and comparison of its chemical composition, combined with the results of morphological identification, it plays the role of assisting in the identification of eucommia seed fossils, and confirms that the fossil is a eucommia seed fossil.

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Abstract

The invention relates to a method for the auxiliary identification of fossils. The method comprises the following steps: chemical components in an extract of a modern relevant species are compared with chemical components in the fossils through adopting a high performance liquid chromatography fingerprint technology, and consistencies of common chromatographic peaks are analyzed to realize the auxiliary identification of the fossils.

Description

field of invention [0001] The invention relates to a method for assisting the identification of fossils by utilizing the chemical composition fingerprint technology. Background technique [0002] "The only way to reproduce the history of the plant kingdom is to study fossil plants in rocks" (written by T.N. Taylor, translated by Li Zhongming. Paleobotany—Introduction to Fossil Plant Biology. Beijing: Science Press, 1992, p1), therefore, plant fossils It is of great significance to study the evolutionary history of plants. The correct identification of fossils is the basic guarantee for studying the history of plant evolution through fossils. If fossils cannot be accurately identified, it is impossible to study plant evolution. Due to the limitation of geological preservation conditions, the plant fossils obtained now are often incomplete, and cannot be comprehensively identified through reproductive organs and vegetative organs like modern plants. And because the genetic m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N33/24
Inventor 王如峰王宇飞吴秀稳安燕南
Owner BEIJING HUAMIAO TRADITIONAL CHINESE MEDICINE ENG TECH DEV CENT