Polarization interference multi-element CARS (Coherent Anti-stokes Raman Scattering) microscopic imaging method
A microscopic imaging and polarization technology, applied in microscopes, optical components, optics, etc., can solve the problems of difficult to use, time-consuming, reducing the contrast and spectral selectivity of resonance imaging, and achieve the effect of eliminating non-resonant background noise.
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[0008] The principle of the present invention is as figure 1 As shown, the startup and working wavelength selection of Ti:Sapphire femtosecond polarized laser 2 is controlled by wavelength adjustment and initiator 1. The working wavelength adjustment range of femtosecond polarized laser 2 is 700~1000nm, the repetition frequency is 80MHz, and the pulse width is 120fs. At the beginning of the measurement, the operating wavelength is selected as 850nm by the wavelength adjustment and initiator 1 and the femtosecond polarized laser 2 is started. After being split by the beam splitter A 3, it is divided into CARS probe light and PCF (photonic crystal fiber) pump light, two The power ratio can be set as 3 to 7. PCF pumping light enters PCF 5 after being focused by microscope objective lens A 4. Due to the optical properties of PCF 5, it can emit supercontinuum laser light, which is expanded by microscope objective lens A' 6 After that, the beam splitter B 7 is divided into two parts...
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