Huperzia serrata propagation method
A technology of Huperzia serrata and seed collection, which is applied in the breeding field of Huperzia serrata, can solve the problems of large-scale reproduction without spores, no fundamental solution to the problems of rapid reproduction of Huperzia serrata and high cost of seedlings
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Embodiment 1
[0019] On October 5, 2 g of mature spores of Huperzia serrata (Thunb.) Trev. were collected and stored at -30°C. Peat is used to make a seedling bed with a width of 60 cm and a thickness of 8 cm, and an equal volume aqueous solution of 0.1 ppm IAA and IBA is used to make the water content of the substrate reach 50%. Take 0.02g of cryopreserved spores and sow them on 1m 2 seedbed. The temperature of the seedbed was controlled at 18°C, the relative air humidity was controlled at 50%, the water holding capacity of the substrate was controlled at 50%, the light intensity was controlled at 500lx, and the number of spores germinated in 173 days was 23879. After the spores germinated, the temperature was controlled at 20°C, the relative air humidity was controlled at 60%, the substrate water holding capacity was controlled at 70%, the light intensity was controlled at 1000lx, and 16954 three-leaf seedlings were formed in 175 days.
example 2
[0021] On October 20, 3 g of mature spores of Huperzia serrata (Thunb.) Trev. were collected and stored at -15°C. Adopt leaf humus to make a seedling raising bed with a width of 65 cm and a thickness of 9 cm, and use 1 ppm IAA and 0.5 ppm IBA aqueous solution to make the water content of the substrate reach 60%. Take 0.01g of cryopreserved spores and sow them on 1m 2 Seedbed. The temperature was controlled at 20°C, the relative air humidity was controlled at 60%, the substrate water holding capacity was controlled at 60%, the light intensity was controlled at 1000 lx, and the number of spores germinated in 168 days was 11089. After germination, the temperature of the seedbed is controlled at 24°C, the relative humidity of the air is controlled at 60%, the water holding capacity of the substrate is controlled at 70%, and the light intensity is controlled at 1000 lx. 8094 three-leaf seedlings can be formed in 178 days.
example 3
[0023] On November 20, 6 g of mature spores of Huperzia serrata (Thunb.) Trev. were collected and stored at a low temperature of 0°C. Use leaf humus to make a seedling bed with a width of 70 cm and a thickness of 10 cm, and use 3 ppm NAA and 1.5 ppm IBA aqueous solution to make the water content of the substrate reach 70%. Take 0.3g of cryopreserved spores and sow them on 1m 2 Seedbed. The temperature was controlled at 22°C, the relative air humidity was controlled at 70%, the substrate water holding capacity was controlled at 70%, the light intensity was controlled at 1500lx, and the number of spores germinated in 155 days was 31453. After the spores germinate, the temperature is controlled at 24°C, the relative air humidity is controlled at 70%, the water holding capacity of the substrate is controlled at 80%, and the light intensity is controlled at 1500lx. 23589 3-leaf seedlings can be formed in 178 days.
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