Saccharomyces cerevisiae deleted bacterial strain and application thereof in furfural resistant aspect
A technology of Saccharomyces cerevisiae and strains, applied in the direction of fermentation, fungi, and microorganism-based methods, etc., can solve the problems of reducing ethanol production efficiency, achieve large scale, high efficiency, and improve tolerance
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Embodiment 1
[0021] Example 1 Furfural resistance experiment of Saccharomyces cerevisiae deletion strain KKSC1
[0022] Take the Saccharomyces cerevisiae deletion strain KKSC1 and culture it overnight at 30°C in liquid YPD medium for 8-12 hours, and then adjust the resulting culture to OD600 nm With a value of 2.0, the culture was obtained for use.
[0023] YPD medium: yeast extract 10g / L, peptone 20g / L, glucose 20g / L.
[0024] Take the obtained culture and wild-type Saccharomyces cerevisiae strain, dilute them 5 times with distilled water as the solution respectively according to ten times the concentration, and obtain 5 cell dilution solutions with decreasing concentrations accordingly, and label them respectively for future use.
[0025] Take 3 μL of 5 diluted cell solution samples from KKSC1 and wild-type Saccharomyces cerevisiae strains, respectively, and inoculate them on SC solid medium without or with 10 mM furfural, and culture them on plates at 30°C for 48 hours.
[0026] SC so...
Embodiment 2
[0029] Example 2 Furfural resistance experiment of Saccharomyces cerevisiae deletion strain KKSC2
[0030] Take the Saccharomyces cerevisiae deletion strain KKSC2 and culture it overnight at 30°C in liquid YPD medium for 8-12 hours, and then adjust the resulting culture to OD600 nm With a value of 2.0, the culture was obtained for use.
[0031] YPD medium: yeast extract 10g / L, peptone 20g / L, glucose 20g / L.
[0032] Take the obtained culture and wild-type Saccharomyces cerevisiae strain, use distilled water as the solution, and dilute them 5 times according to the concentration of 10 times successively to obtain 5 cell dilution solutions with decreasing concentrations accordingly, and label them separately for future use.
[0033] Take 3 μL of 5 diluted cell solution samples from KKSC2 and wild-type Saccharomyces cerevisiae strains and spot them on SC solid medium without or with 10 mM furfural, and culture them on plates at 30°C for 48 hours.
[0034] SC solid medium: glucos...
Embodiment 3
[0036] Example 3 Furfural resistance experiment of Saccharomyces cerevisiae deletion strain KKSC3
[0037] Take the Saccharomyces cerevisiae deletion strain KKSC3 and culture it overnight in liquid YPD medium at 30°C for 8-12 hours, then adjust the resulting culture to OD600 nm With a value of 2.0, the culture was obtained for use.
[0038] YPD medium: yeast extract 10g / L, peptone 20g / L, glucose 20g / L.
[0039] Take the obtained culture and wild-type Saccharomyces cerevisiae strain, dilute them 5 times with distilled water as the solution respectively according to ten times the concentration, and obtain 5 cell dilution solutions with decreasing concentrations accordingly, and label them respectively for future use.
[0040] Take 3 μL of 5 diluted cell solution samples from KKSC3 and wild-type Saccharomyces cerevisiae strains, respectively, and spot them on SC solid medium without or with 10 mM furfural, and culture them on plates at 30°C for 48 hours.
[0041] SC solid medium:...
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