Method for producing alcohol by mixing and fermenting raw materials and cooked materials
A technology of mixed fermentation and alcohol is applied in the field of mixed fermentation of raw meal and clinker to produce alcohol. Fast, easy-to-use effects
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Embodiment 1
[0026] 1): Take 50g of corn flour as the starchy raw material, and divide it into two parts: one part is 40g as raw material I, and the other part is 10g as raw material II.
[0027] a. Take 40g of corn flour as raw material I, add 100g (100ml) of water, that is, the ratio of raw material I to water is 1:2.5, add 8U / g raw material high-temperature liquefied enzyme, cook in a pressure cooker at 105°C for 1 hour, and cool down To 60°C, add 200U / g starchy raw material saccharification enzyme, and keep warm for saccharification for 1 hour.
[0028] b. Take 10g of corn flour as raw material II, add 25g of water, that is, the material-water ratio of raw material II to water is 1:2.5, and add 8U / g of medium-temperature liquefaction enzyme of raw material.
[0029] 2): Mix the above materials.
[0030] 3): Finally, synchronous saccharification and fermentation to produce alcohol: add 200U / g starchy raw material glucoamylase and 0.02% neutral protease based on the weight of raw materi...
Embodiment 2
[0035] 1): Take 100g of corn flour as the starchy raw material, and divide it into two parts: one part is 60g as raw material I, and the other part is 40g as raw material II.
[0036] a. Take 60g of corn flour as raw material I, add 180g (180ml) of water, add 10U / g high-temperature liquefied enzyme of raw material, cook in a pressure cooker at 105°C for 1 hour, cool down to 60°C, and add 200U / g based on the weight of raw material I Glucoamylase for starchy raw materials, heat preservation and saccharification for 1 hour. Then cool down to 32°C.
[0037] b. Take corn flour as raw material II of 40g, add 120g (120ml) of water, add 10U / g of medium temperature liquefaction enzyme of raw material, add 300U / g of glucoamylase of starchy raw material and 0.01% of acid protease based on the weight of raw material II , heat preservation at 60°C for liquefaction and saccharification for 2 hours, and then lower the temperature to 32°C.
[0038] 2): Add the material obtained in step b to...
Embodiment 3
[0044] 1): Weigh 400g of cassava flour and 200g of corn flour as starchy raw materials, of which 400g of cassava flour is used as raw material I, and 200g of corn flour is used as raw material II.
[0045] a. Take 400g of cassava flour raw material I, add 1000g (1L) of water, add 10U / g of raw material high-temperature liquefied enzyme, and cook in a pressure cooker at 105°C for 1 hour.
[0046] b. Take 200g of corn flour raw material II, add 500g (500ml) of water, and add 10U / g of raw material medium temperature liquefaction enzyme.
[0047] 2): Add all the materials obtained in step b to the materials obtained in step a, adjust the temperature to 60°C, add 160U / g starchy raw material glucoamylase calculated based on 400g cassava flour, and 300U / g starch calculated based on 200g corn flour Glucoamylase of high-quality raw materials, and keep warm for saccharification for 1 hour.
[0048] 3): Finally, synchronous saccharification and fermentation to produce alcohol: after cool...
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