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Method for measuring chlorophyll a in water

A technology for the determination of chlorophyll and water, which is applied in the preparation of test samples and the measurement of color/spectral characteristics. It can solve the problems of poor measurement stability, poor control of hydrochloric acid usage, and poor reproducibility, so as to achieve complete extraction and avoid chlorophyll. Loss, good reproducibility effect

Inactive Publication Date: 2012-07-18
HARBIN INST OF TECH
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Problems solved by technology

[0005] The present invention aims to solve the problems that the existing ethanol freezing method for measuring chlorophyll-a requires acidification with hydrochloric acid, and the amount of hydrochloric acid used is not easy to control, resulting in poor measurement stability and poor reproducibility, and provides a method for measuring chlorophyll-a in water

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  • Method for measuring chlorophyll a in water
  • Method for measuring chlorophyll a in water
  • Method for measuring chlorophyll a in water

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specific Embodiment approach 1

[0010] Specific embodiment one: the method for measuring chlorophyll a in water in this embodiment is carried out according to the following steps:

[0011] 1. Take 50-200ml of water sample, filter it with 0.45μm cellulose acetate filter membrane, control the vacuum pump pressure at 0.05MPa, and obtain the filter membrane with retained algae cells; 2. Cut the filter membrane with retained algal cells into pieces Put 6ml of ethanol solution with a volume concentration of 90% in a centrifuge tube, and refrigerate at 4°C for 12 to 24 hours; 3. Then centrifuge the centrifuge tube at 8000r / min for 15min, collect the supernatant, and then add 4ml to the centrifuge tube The ethanol solution with a volume concentration of 90% was centrifuged at 8000r / min for 5min, the supernatant was collected, and the supernatant obtained by two centrifuges were combined, and the ethanol solution with a mass concentration of 90% was added to dilute to 10ml to obtain the extract Four, the extract is c...

specific Embodiment approach 2

[0013] Specific embodiment two: the difference between this embodiment and specific embodiment one is: take 50ml water sample in step one. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0014] Specific embodiment three: the difference between this embodiment and specific embodiment one is: take 200ml water sample in step one. Others are the same as in the first embodiment.

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Abstract

The invention relates to a method for measuring chlorophyll a in water, solving the problems that the measuring stability is poor and the repeatability is poor, which are caused by a situation that the usage amount of hydrochloric acid is not easy to control, wherein the hydrochloric acid needs to be acidized in the conventional ethanol freezing method for measuring chlorophyll a; the method comprises the following steps: fetching a water sample and filtering the water sample, cutting up a filter film which intercepts frustules, putting the cut filter film in ethanol solution, performing centrifugation on the ethanol solution after refrigeration, collecting supernatant liquid, supplementing ethanol solution, performing centrifugation on the ethanol solution, collecting supernatant liquid, mixing the supernatant liquids, and adding ethanol solution to the supernatant liquids until the constant volume is 10ml, thereby obtaining extract liquid; and performing centrifugation on the extract liquid to obtain supernatant liquid of the extract liquid, measuring the absorbance values of the supernatant liquid of the extract liquid under the wavelengths of 630nm, 647nm, 664nm and 750nm, and calculating the content of the chlorophyll a in water. The method provided by the invention does not need grinding, manpower is greatly saved, the loss of chlorophyll caused by transferring of the extract liquid is avoided, the influence of the acidification degree of the hydrochloric acid is avoided, extraction is complete and repeatability is good.

Description

technical field [0001] The invention relates to a method for measuring chlorophyll a in water. Background technique [0002] The assay method of chlorophyll in the water body mainly contains the acetone grinding method recommended in " water and waste water monitoring and analysis method " (fourth edition) published by the State Environmental Protection Agency to measure chlorophyll a at present, and this method is to filter the water sample with a cellulose acetate filter membrane, After the filter membrane retaining algae is dried at low temperature for 6-8 hours, add 90% acetone to repeatedly grind and extract, centrifuge, measure and calculate the chla content with a spectrophotometer. The disadvantage of this method is that 1. during repeated grinding and extraction, chlorophyll is exposed to the environment and is prone to photolysis; And the volatility is strong, which is unfavorable to the health of the staff; 4. During repeated solvent addition and repeated transfe...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N1/42
Inventor 张素春舒坦杜茂安安众一杨雪
Owner HARBIN INST OF TECH
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