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Method for identifying liquorice in Huoxiang Zhengqi oral liquid

The technology of Zhengqi Oral Liquid and Huoxiang is applied in measuring devices, instruments, scientific instruments and other directions, which can solve the problems of poor specificity of identification methods and negative interference.

Active Publication Date: 2012-07-18
BEIJING ASIA EAST BIO PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above two methods, licorice uses chloroform (i.e. chloroform) as a solvent for identification, and extraction with chloroform has negative interference, and the specificity of the identification method is poor

Method used

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  • Method for identifying liquorice in Huoxiang Zhengqi oral liquid
  • Method for identifying liquorice in Huoxiang Zhengqi oral liquid
  • Method for identifying liquorice in Huoxiang Zhengqi oral liquid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1: " Chinese Pharmacopoeia " 2010 edition one 1231 pages of identification (4)

[0041] 1. Reagents and reagents

[0042] Huoxiangzhengqi Oral Liquid: Beijing Yadong Biopharmaceutical Co., Ltd., batch numbers: 1107003, 1107004, 1107005. Taiji Group Chongqing Fuling Pharmaceutical Factory Co., Ltd., batch numbers: 10121254, 11020087, 11050113.

[0043] Reference substance and reference medicinal materials: ammonium glycyrrhizinate reference substance (for content determination, provided by China Institute for the Control of Pharmaceutical and Biological Products), batch number: 110731-200615; licorice reference substance (for identification and determination, provided by China National Institute for the Control of Pharmaceutical and Biological Products), batch number : 120904-200914.

[0044] 2. The original identification method:

[0045] 1) Take Huoxiangzhengqi Oral Liquid 30ml, shake and extract 2 times with ether, 10ml each time, discard the ether solut...

Embodiment 2

[0051] Embodiment 2: improve standard

[0052] 1. Acidification with hydrochloric acid before n-butanol extraction

[0053] 1.1 Acidification:

[0054] 1) Take Huoxiangzhengqi Oral Liquid 30ml, shake and extract with diethyl ether twice, 10ml each time, discard the ether solution, add 1ml hydrochloric acid, shake well, shake and extract 3 times with n-butanol saturated with water, 10ml each time, Combine the n-butanol solution, wash with water twice, 10ml each time, discard the water solution, evaporate the n-butanol solution to dryness, add 2ml methanol to the residue to dissolve, and use it as the test solution②.

[0055] 2) Take another 1g of licorice reference medicinal material, add 20ml of ether, heat and reflux for 15 minutes, filter, discard the ether solution, evaporate the solvent from the medicinal residue, add 20ml of methanol, ultrasonicate for 30 minutes, filter, evaporate the filtrate to dryness, add water to the residue 20ml to dissolve, add 1ml of hydrochlor...

Embodiment 3

[0073] Embodiment 3: the identification method of licorice in Huoxiangzhengqi oral liquid

[0074] 1. Identification method:

[0075] 1) Take 5ml of Huoxiangzhengqi Oral Liquid, add 5ml of water, shake well, shake and extract with diethyl ether twice, 10ml each time, discard the ether solution, add 0.2ml of hydrochloric acid, shake well, shake and extract with n-butanol saturated with water 3 times, 10ml each time, combined n-butanol solution, washed with water 2 times, 10ml each time, discarded the water solution, evaporated the n-butanol solution to dryness, added 2ml methanol to the residue to dissolve, and used it as the test solution.

[0076] 2) Take another 0.5g of licorice reference medicinal material, add 20ml of ether, heat and reflux for 15 minutes, filter, discard the ether solution, evaporate the solvent from the medicinal residue, add 20ml of methanol, ultrasonicate for 30 minutes, filter, and evaporate the filtrate to dryness. Add 10ml of water to dissolve, add...

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Abstract

The invention relates to a method for identifying liquorice in Huoxiang Zhengqi oral liquid. The method comprises the following steps of: preparing tested sample solution; preparing control medicine solution; preparing control product solution; and performing thin layer chromatographic analysis, wherein the sampling amount of the tested sample is 5 ml; 5 ml of the tested sample is diluted by 5 ml of water; the sampling amount of the control medicine is 0.5 g; and in the preparation process of the tested sample solution and the control medicine solution, 0.2 ml of hydrochloric acid is added to perform acidification before extraction by normal butanol. In the conventional identification method, no corresponding spot exists or the spot is weak, so that people cannot see clearly, the reproducibility is low, and the assay results of the products with the same quality detected by different staff and different labs are different. Compared with the conventional identification method, the identification method has the advantages of clear identification spots, high reproducibility and high specificity.

Description

technical field [0001] The invention relates to a method for quality control of active ingredients in Chinese patent medicines, in particular to a method for identifying licorice in Huoxiangzhengqi oral liquid. Background technique [0002] Huoxiangzhengqi San is one of the famous prescriptions through the ages. Its function is to relieve the exterior and remove dampness, regulate qi and harmonize the middle. Indications: Exogenous wind and cold in summer and autumn, internal injury and stagnation of dampness, manifested as chills, headache, vomiting, diarrhea, abdominal distension, thick tongue coating, a good remedy for summer colds. [0003] Huoxiang Zhengqi Oral Liquid, which is improved from the traditional Chinese medicine Huoxiang Zhengqi Water, is a pure Chinese medicine preparation made according to the theory of traditional Chinese medicine. For colds caused by exogenous wind-cold, internal injury damp stagnation or summer injury and summer dampness. Symptoms incl...

Claims

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Application Information

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IPC IPC(8): G01N30/90
Inventor 付立家付建家马云赵敏姿
Owner BEIJING ASIA EAST BIO PHARMA CO LTD
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