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ACL (ATP (Adenosine Triphosphate) citrate lyase) gene relative to intramuscular fat deposition

An intramuscular fat and gene technology, applied in genetic engineering, plant genetic improvement, biochemical equipment and methods, etc., can solve the problem of little understanding of the molecular mechanism of Banna miniature pigs

Inactive Publication Date: 2012-07-25
YUNNAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, little is known about the growth and development and molecular mechanisms of fat deposition in Banna minipigs

Method used

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  • ACL (ATP (Adenosine Triphosphate) citrate lyase) gene relative to intramuscular fat deposition
  • ACL (ATP (Adenosine Triphosphate) citrate lyase) gene relative to intramuscular fat deposition
  • ACL (ATP (Adenosine Triphosphate) citrate lyase) gene relative to intramuscular fat deposition

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Embodiment Construction

[0018] An intramuscular fat deposition ACL gene is an ATP citrate lyase (ATP citrate lyase, ACL) gene related to intramuscular fat deposition isolated and cloned from the muscle tissue of Banna miniature pigs. The full length of the CDS sequence of the gene is 3276bp, and its CDS nucleotide sequence is shown in the nucleotide and amino acid sequence table of SEQ.ID.NO.1. In addition, the CDS nucleotide sequence encodes 1091 amino acids, and the amino acid sequence is as shown in SEQ.ID.NO.2 in the specification nucleotide and amino acid sequence table.

[0019] Primers were designed for the ACL gene with the above characteristics of intramuscular fat deposition, and PCR amplification was performed to detect the tissue expression profile of the ACL gene in seven tissues of pig heart, liver, spleen, lung, kidney, muscle and fat. The expression level was the highest in tissues, preliminarily indicating that ACL gene regulates the function of fat deposition in adipocytes.

[0020...

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Abstract

The invention discloses an ACL (ATP (Adenosine Triphosphate) citrate lyase) gene relative to intramuscular fat deposition. The ACL gene is separated and cloned from a muscular tissue of a Bannan miniature pig and is relative to the intramuscular fat deposition. The overall length of the CDS sequence of the gene is 3276bp, the CDS nucleotide sequence of the gene is shown in SEQ.ID.NO.1 and is coded with 1091 amino acids, and the amino acid sequence is shown in SEQ.ID.NO.2. According to detection on tissue expression profiles of the ACL gene in seven tissues, namely the heart, liver, spleen, lungs, kidney, muscle and fat of the pig, the detection result shows that the expression index of the ACL gene in the fat tissue is highest, thereby initially showing the function of the ACL gene for regulating and controlling fat deposition in fat cells.

Description

technical field [0001] The invention relates to the genetic engineering technology of animal molecular breeding, in particular to an ATP citrate lyase (ATP citrate lyase, ACL) gene technology related to pig intramuscular fat deposition. Background technique [0002] At present, while my country's pig production is pursuing high lean meat percentage and low backfat thickness, it ignores the decline of pork quality. The content of intramuscular fat (IMF) is an important factor affecting pork quality, which can affect many aspects of meat quality, such as flavor, juiciness, tenderness and nutritional value. Therefore, increasing the intramuscular fat content to improve pork quality has become a current research hotspot. Although the local breed pigs in my country grow slowly, they have better meat quality. For example, the Banna miniature pig is one of the excellent local pig breeds in Yunnan. It has the characteristics of well-proportioned body, long and fast fat, thin skin ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/60C12N9/88C12Q1/68
Inventor 高士争黄英潘洪彬赵素梅王静王聪刘维全朱宝利
Owner YUNNAN AGRICULTURAL UNIVERSITY
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