Preparation method of oxidation coenzyme I
An oxidized coenzyme and enzyme powder technology, which is applied in the direction of fermentation, etc., can solve the problems of expensive products, restrictions on the production and development of nicotinamide adenine dinucleotide, energy consumption and large material consumption, and achieve high conversion rate and good industrialization Application prospects, the effect of avoiding high energy consumption
Active Publication Date: 2013-04-17
ENZYMEWORKS
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Problems solved by technology
Although the process is mature, it consumes a lot of energy and materials, and the product is expensive, which limits the production of nicotinamide adenine dinucleotide and the development of its subsequent application process
Method used
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Embodiment 2
[0020] The above-mentioned embodiments are only to illustrate the technical concept and characteristics of the present invention, and the purpose is to enable those skilled in the art to understand the content of the present invention and implement it accordingly, and not to limit the protection scope of the present invention. All equivalent changes or modifications made according to the spirit of the present invention shall fall within the protection scope of the present invention.
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The invention relates to a preparation method of an oxidation coenzyme I. According to the method, nicotinamide ribonucleoside (NR) and adenosine disodium triphosphate (ATP-Na2) react with each other to obtain the oxidation coenzyme I (NAD+, Nicotinamide Adenine Dinucleotide+) in a buffer solution with the pH of 5.0 to 8.0 at a temperature of 30 DEG C to 40 DEG C under the catalytic action of nicotinamide ribonucleoside kinase (NRK) under the condition of the existence of divalent metal ions. According to the invention, a biocatalysis method is adopted, the oxidation coenzyme is prepared by utilizing an NRK one pot process, the reaction system is simple, the conditions are mild, and the preparation method has wide industrialization application prospect.
Description
technical field [0001] The invention relates to a preparation method of oxidized coenzyme I. Background technique [0002] Among all the enzymes discovered so far, about 30-35% are oxidoreductases. In industrial biotechnology, especially in biopharmaceutical and fine chemical industries, oxidoreductases are the most important enzymes. In the reactions catalyzed by oxidoreductases, certain coenzymes need to be consumed for the transfer of electrons and protons while producing products. According to statistics, about 80% of the reactions require nicotinamide adenine dinucleotide oxidized coenzyme I (abbreviated as NAD+, see formula I for structural formula) as a coenzyme, and about 10% of the reactions require nicotinamide adenine dinucleotide phosphate That is, oxidized coenzyme II (NADP+ for short) is used as a coenzyme, and only a small amount of reactions require flavin (FAD, FMN) and coenzyme Q (PQQ) as a coenzyme. Although the addition of a complex coenzyme regenerati...
Claims
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IPC IPC(8): C12P19/32
Inventor 陶军华李斌鞠鑫梁晓亮庄季昌
Owner ENZYMEWORKS