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Non-invasive prenatal detection kit for down syndrome

A technology for Down syndrome and kit, applied in the field of molecular biology, can solve problems such as difficulty in testing

Inactive Publication Date: 2012-07-25
解码(上海)生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, its sensitivity and reliability in detecting different fetal malformation symptoms have yet to be confirmed, and due to the small number of embryonic cells, there are still great difficulties in testing

Method used

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  • Non-invasive prenatal detection kit for down syndrome
  • Non-invasive prenatal detection kit for down syndrome
  • Non-invasive prenatal detection kit for down syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1. Use of detection kits

[0023] 1. Acquisition of DNA

[0024] DNA fragments extracted from isolated peripheral blood of pregnant women.

[0025] The isolated peripheral plasma of pregnant women was obtained, and the cell-free DNA in the plasma was extracted using the kit QIAampDNAMidireagentset (Qiagen, catlog #51183) by using the "blood and body fluid" protocol in the kit. The purification process of DNA was carried out automatically on QIAcube (Qiagen9001292) using theQIAampDNABloodMiniKit kit, and this step can be automatically completed by these kits. .

[0026] 2. End repair

[0027] DNA extracted from the blood of pregnant women is double-stranded DNA fragments that are blunt-ended or contain 3 or 5' overhangs. In this step, the overhangs are phosphorylated to blunt ends by T4 DNA polymerase, E. coli DNA polymerase I large fragment (Klenow fragment), and polynucleotide kinase T4. 3' to 5' exonuclease activity removes the 3' overhang and polymerase ...

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Abstract

The invention provides a non-invasive prenatal detection kit for down syndrome, which comprises a T4 DNA ligase buffer solution, a Klenow fragment (5 units / mul), T4 polynucleotide kinase (T4PNK), an linker sequence, a DNA (deoxyribonucleic acid) sequencing primer, a PCR (polymerase chain reaction) component, a DNA sequencing component and the like. Through establishing an in vitro DNA library from peripheral blood of a pregnant woman, the kit can be used for accurate sequencing a genomic sequence of a fetus and screening the down syndrome of the fetus, with accurate results, and without false positive and false negative. Furthermore, the kit can be used for detection of a small number of in vitro peripheral blood samples, so as to greatly reduce the pain and risk of the pregnant woman, and the kit has good application prospects.

Description

technical field [0001] The invention belongs to the field of molecular biology, relates to medicine and biotechnology, and specifically relates to a prenatal non-invasive detection kit for Down syndrome. The diagnostic kit can perform early detection and diagnosis on pregnant women after the ninth week. Results From the genetic level, it can be accurately diagnosed whether the fetus has Down syndrome. Background technique [0002] A small number of newborns have congenital genetic diseases or birth defects, which bring huge economic and spiritual burdens to society and families. In 2007, 15.64 million newborns were born in China, and the birth defect rate exceeded 1%. Birth defects are an important issue affecting the health level of the whole people and the quality of the population in our country. The national "Eleventh Five-Year Plan" listed reproductive health-related technologies as a priority theme for scientific and technological development; the national medium and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 潘加奎伍建姜丽
Owner 解码(上海)生物医药科技有限公司
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