Salbutamol enzyme linked immunoassay kit and use method thereof

Abstract

The invention relates to a salbutamol enzyme linked immunoassay kit and a use method of the salbutamol enzyme linked immunoassay kit. The salbutamol enzyme linked immunoassay kit comprises the following components: a coated microplate, calibrator, enzyme label working solution, substrate solution A, substrate solution B, stop buffer, concentrated wash solution and concentrated extracting solution. The kit prepared according to the invention can be used for quickly quantifying and detecting salbutamol residual amount in samples such as urine or serum, muscle or tissue, feed, milk and the like, and has the advantages of wide use range, convenience in use and accurate detection. The sensitivity of the kit prepared according to the invention is 0.1 ppb; for the precision, the within-run coefficient of variation (CV%) is less than 10 percent, and the between-run coefficient of variation (CV%) is less than 15 percent; the accuracy is represented as recovery rate, and the recovery rate should be 70-110 percent. The range of IC50 is 0.6-1.0 ppb, and the absolute value of the linearly dependent coefficient r is not less than 0.9900.

Description

technical field [0001] The invention belongs to the field of detection kits, and relates to a stimulant detection kit, in particular to a salbutamol ELISA kit and a use method thereof. Background technique [0002] Salbutamol (Salbutamol, SAL) belongs to a kind of β-2 stimulant, is a kind of drug that can bind to β-2 adrenergic receptor, and is mainly used for treating asthma and bronchospasm clinically. The use of SAL as a feed additive can significantly promote animal growth, increase feed conversion rate and lean meat rate, and if used in excess for a long time, it will accumulate in animal tissues, and human consumption of this animal product will cause poisoning. [0003] Clenbuterol (CL) is most commonly used as a growth-promoting agent among β-2 stimulants, but as the detection of CL increases, illegal users begin to turn to other alternatives. Since the growth-promoting effects of SAL and CL are not much different, its elimination time in animals is shorter than tha...

AI Technical Summary

Problems solved by technology

[0006] 3. The harm of salbutamol animal food residues to human body

However, both HPLC and GC / MS methods require expensive instruments, complicated sample processing procedures, and special operating skills, and are time-consuming, so they are not suitable for the detection of a large number of samples, but their effectiveness, accuracy, and sensitivity make them It still has advantages that cannot be replaced by ELISA method

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